We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC00946Purine and other phosphorylases family 1 signature
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PURL: https://purl.expasy.org/prosite/documentation/PDOC00946
Description
The following phosphorylases belongs to the same family:
- Purine nucleoside phosphorylase (EC 2.4.2.1) (PNP) from most bacteria (gene deoD). This enzyme catalyzes the cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [1].
- Uridine phosphorylase (EC 2.4.2.3) (UdRPase) from bacteria (gene udp) and mammals. Catalyzes the cleavage of uridine into uracil and ribose-1- phosphate. The products of the reaction are used either as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis [2].
- 5'-methylthioadenosine phosphorylase (EC 2.4.2.28) (MTA phosphorylase) from Sulfolobus solfataricus [3].
As a signature pattern, we selected a conserved region in the central part of these enzymes.
Note:It should be noted that mammalian and some bacterial PNP as well as eukaryotic MTA phosphorylase belong to a different family of phosphorylases (see <PDOC00954>).
Last update:December 2004 / Pattern and text revised.
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Technical section
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References
| 1 | Authors | Takehara M. Ling F. Izawa S. Inoue Y. Kimura A. |
| Title | Molecular cloning and nucleotide sequence of purine nucleoside phosphorylase and uridine phosphorylase genes from Klebsiella sp. | |
| Source | Biosci. Biotechnol. Biochem. 59:1987-1990(1995). | |
| PubMed ID | 8534998 |
| 2 | Authors | Watanabe S.-I. Hino A. Wada K. Eliason J.F. Uchida T. |
| Title | Purification, cloning, and expression of murine uridine phosphorylase. | |
| Source | J. Biol. Chem. 270:12191-12196(1995). | |
| PubMed ID | 7744869 |
| 3 | Authors | Cacciapuoti G. Porcelli M. Bertoldo C. De Rosa M. Zappia V. |
| Title | Purification and characterization of extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus. Purine nucleoside phosphorylase activity and evidence for intersubunit disulfide bonds. | |
| Source | J. Biol. Chem. 269:24762-24769(1994). | |
| PubMed ID | 7929153 |
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