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Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC00954Purine and other phosphorylases family 2 signature
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PURL: https://purl.expasy.org/prosite/documentation/PDOC00954
Description
The following phosphorylases belongs to the same family:
- Purine nucleoside phosphorylase (EC 2.4.2.1) (PNP) from mammals as well as from some bacteria (gene deoD). This enzyme catalyzes the cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [1].
- 5'-methylthioadenosine phosphorylase (EC 2.4.2.28) (MTA phosphorylase) from eukaryotes [2].
- Xanthosine phosphorylase (EC 2.4.2.-) from Escherichia coli (gene xapA). This enzyme can degrade all purine nucleosides except adenosine and deoxyadenosine [3].
This family also includes the following uncharacterized proteins:
- Yeast hypothetical protein YLR017w.
- Fission yeast hypothetical protein SpAC16C9.02c.
- Methanococcus jannaschii hypothetical protein MJ0060.
- Rhodospirillum rubrum hypothetical protein in petC 3'region.
As a signature pattern, we selected a conserved region in the central part of these enzymes.
Note:It should be noted that most bacterial PNP as well as archaebacterial MTA phosphorylase belong to a different family of phosphorylases (see <PDOC00946>).
Last update:December 2004 / Pattern and text revised.
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Technical section
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References
| 1 | Authors | Ealick S.E. Rule S.A. Carter D.C. Greenhough T.J. Babu Y.S. Cook W.J. Habash J. Helliwell J.R. Stoeckler J.D. Parks R.E. Jr. Chen S.-F. Bugg C.E. |
| Title | Three-dimensional structure of human erythrocytic purine nucleoside phosphorylase at 3.2 A resolution. | |
| Source | J. Biol. Chem. 265:1812-1820(1990). | |
| PubMed ID | 2104852 |
| 2 | Authors | Della Ragione F. Takabayashi K. Mastropietro S. Mercurio C. Oliva A. Russo G.L. Della Pietra V. Borriello A. Nobori T. Carson D.A. Zappia V. |
| Title | Purification and characterization of recombinant human 5'-methylthioadenosine phosphorylase: definite identification of coding cDNA. | |
| Source | Biochem. Biophys. Res. Commun. 223:514-519(1996). | |
| PubMed ID | 8687427 |
| 3 | Authors | Seeger C. Poulsen C. Dandanell G. |
| Title | Identification and characterization of genes (xapA, xapB, and xapR) involved in xanthosine catabolism in Escherichia coli. | |
| Source | J. Bacteriol. 177:5506-5516(1995). | |
| PubMed ID | 7559336 |
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