PROSITE documentation PDOC00962Hydrogenases expression/synthesis hypA family signature
Bacterial membrane-bound nickel-dependent hydrogenases requires a number of accessory proteins which are involved in their maturation. The exact role of these proteins is not yet clear, but some seem to be required for the incorporation of the nickel ions [1,2]. One of these proteins is generally known as hypA. It is a protein of about 12 to 14 Kd that contains, in its C-terminal region four conserved cysteines that may bind nickel.
Escherichia coli has two proteins that belong to this family: hypA and hybF. Homolog have also been found in the genome of many archaea.
We developed a signature pattern that starts about 45 residues before the first conserved cysteine and that ends with the second one.
Last update:April 2006 / Pattern revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Fu C. Maier R.J. |
| Title | Nucleotide sequences of two hydrogenase-related genes (hypA and hypB) from Bradyrhizobium japonicum, one of which (hypB) encodes an extremely histidine-rich region and guanine nucleotide-binding domains. | |
| Source | Biochim. Biophys. Acta 1184:135-138(1994). | |
| PubMed ID | 8305450 |
| 2 | Authors | Olson J.W. Mehta N.S. Maier R.J. |
| Title | Requirement of nickel metabolism proteins HypA and HypB for full activity of both hydrogenase and urease in Helicobacter pylori. | |
| Source | Mol. Microbiol. 39:176-182(2001). | |
| PubMed ID | 11123699 |
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