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	PROSITE documentation PDOC00964

Endogenous opioids neuropeptides precursors signature

Vertebrate endogenous opioids neuropeptides are released by post-translational proteolytic processing of precursor proteins. These precursor proteins consist of a signal sequence followed by a conserved region of about 50 residues, a variable length region and the sequence of the various neuropeptides. Three types of precursors are currently known:

• Preproenkephalin A (gene PENK) which is processed to produce the peptides Met-enkephalin (6 copies) and Leu-enkephalin,
• Preproenkephalin B (gene PDYN) which is processed to produce the peptides neoendorphin, dynorphin, leumorphin, rimorphin and leu-enkephalin.
• Prepronocipeptin (gene PNOC) which is processed to produce the peptides nociceptin (orphanin FQ) as well as two other potential neuropeptides.

The conserved region in the N-terminal of these precursors contains six cysteines that are probably involved in disulfide bonds. This region could be important for the processing of the neuropeptides [1].

Last update:

May 2004 / Text revised.

PROSITE method (with tools and information) covered by this documentation:

OPIOIDS_PRECURSOR, PS01252; Endogenous opioids neuropeptides precursors signature  (PATTERN)

• Consensus pattern:
  C-x(3)-C-x(2)-C-x(2)-[KRH]-x(6,7)-[LIF]-[DNS]-x(3)-C-x-[LIVM]-[EQ]-C-[EQ]-x(8)-W-x(2)-C
  The 6 C's may be involved in disulfide bonds
• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 21
  • detected by PS01252: 17 (true positives)
  • undetected by PS01252: 4 (1 false negative and 3 'partials')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS01252:
  NONE.
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01252
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01252
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS01252
• View ligand binding statistics of PS01252

1	Authors	Mollereau C., Simons M.-J., Soularue P., Liners F., Vassart G., Meunier J.-C., Parmentier M.
	Title	Structure, tissue distribution, and chromosomal localization of the prepronociceptin gene.
	Source	Proc. Natl. Acad. Sci. U.S.A. 93:8666-8670(1996).
	PubMed ID	8710928

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