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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
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Amos Bairoch

PROSITE documentation PDOC00985
Protein-L-isoaspartate(D-aspartate) O-methyltransferase signature


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PURL: https://purl.expasy.org/prosite/documentation/PDOC00985

Description

Protein-L-isoaspartate(D-aspartate) O-methyltransferase (EC 2.1.1.77) (PCMT) [1] (which is also known as L-isoaspartyl protein carboxyl methyltransferase) is an enzyme that catalyzes the transfer of a methyl group from S-adenosylmethionine to the free carboxyl groups of D-aspartyl or L-isoaspartyl residues in a variety of peptides and proteins. The enzyme does not act on normal L-aspartyl residues L-isoaspartyl and D-aspartyl are the products of the spontaneous deamidation and/or isomerization of normal L-aspartyl and L-asparaginyl residues in proteins. PCMT plays a role in the repair and/or degradation of these damaged proteins; the enzymatic methyl esterification of the abnormal residues can lead to their conversion to normal L-aspartyl residues.

PCMT is a well-conserved and widely distributed cytosolic protein of about 24 Kd. As a signature pattern, we selected a conserved region in the central part of this enzyme.

Last update:

December 2004 / Pattern and text revised.

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Technical section

PROSITE method (with tools and information) covered by this documentation:

PCMT, PS01279; Protein-L-isoaspartate(D-aspartate) O-methyltransferase signature  (PATTERN)


Reference

1AuthorsKagan R.M. McFadden H.J. McFadden P.N. O'Connor C. Clarke S.
TitleMolecular phylogenetics of a protein repair methyltransferase.
SourceComp. Biochem. Physiol. 117b:379-385(1997).



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