PROSITE documentation PDOC50186DEP domain profile
The DEP domain is a globular domain of about 80 residues that is found in more than 50 proteins involved in G protein signaling pathways. It has been named after the three proteins (Dishevelled, Egl-10, and Pleckstrin) in which it was initially identified [1]. It has been proposed that the DEP domain could play a selective role in targeting DEP domain-containing proteins to specific subcellular membranous sites, perhaps even to specific G protein-coupled signaling pathways [2,3].
Nuclear magnetic resonance spectroscopy has revealed that the DEP domain comprises a three-helix bundle, a β-hairpin 'arm' composed of two β-strands and two short β-strands in the C-terminal region [3].
Representative example of proteins containing DEP domains are listed below:
- Drosophila and vertebrate dishevelled (Dsh and Dvl), proteins that play a key role in the transduction of the Wg/Wnt signal from the cell surface to the nucleus.
- Vertebrate Pleckstrin, the major substrate of protein kinase C in platelets. Pleckstrin contains two PH domains (see <PDOC50003>) flanking the DEP domain.
- Mammalian regulator of G-protein signaling (RGS) proteins 6, 7, 9 and 11 (see <PDOC50132>).
- Caenorhabditis elegans egl-10, which regulates G protein signaling in nervous system.
The profile we developed covers the entire DEP domain.
Last update:December 2001 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Ponting C.P. Bork P. |
| Title | Pleckstrin's repeat performance: a novel domain in G-protein signaling? | |
| Source | Trends Biochem. Sci. 21:245-246(1996). | |
| PubMed ID | 8755244 |
| 2 | Authors | Burchett S.A. |
| Title | Regulators of G protein signaling: a bestiary of modular protein binding domains. | |
| Source | J. Neurochem. 75:1335-1351(2000). | |
| PubMed ID | 10987813 |
| 3 | Authors | Wong H.C. Mao J. Nguyen J.T. Srinivas S. Zhang W. Liu B. Li L. Wu D. Zheng J. |
| Title | Structural basis of the recognition of the dishevelled DEP domain in the Wnt signaling pathway. | |
| Source | Nat. Struct. Biol. 7:1178-1184(2000). | |
| PubMed ID | 11101902 | |
| DOI | 10.1038/82047 |
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