|PROSITE documentation PDOC50191|
The CRAL-TRIO domain is a structurally conserved element of about 170 amino acids, which constitute a hydrophobic lipid binding pocket. The CRAL-TRIO domain is found in GTPase-activating proteins (GAPs), guanine nucleotide exchange factors (GEFs) and a family of hydrophobic ligand binding proteins, including the yeast SEC14 protein and mammalian retinaldehyde- and α-tocopherol-binding proteins. The CRAL-TRIO domain may either constitute all of the protein or only part of it [1,2,3,4,5].
The resolution of the crystal structure of SEC14 has revealed the structure of the CRAL-TRIO lipid binding domain (see <PDB:1AUA>). The CRAL-TRIO lipid binding domain is an α/β domain, which forms a large hydrophobic pocket. The pocket floor is constituted by six β-strands and the sides of the cavity are formed by α-helices .
Some proteins known to contain a CRAL-TRIO domain are listed below:
The profile we developed covers the entire CRAL-TRIO domain.Last update:
June 2002 / First entry.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Salama S.R. Cleves A.E. Malehorn D.E. Whitters E.A. Bankaitis V.A.|
|Title||Cloning and characterization of Kluyveromyces lactis SEC14, a gene whose product stimulates Golgi secretory function in Saccharomyces cerevisiae.|
|Source||J. Bacteriol. 172:4510-4521(1990).|
|2||Authors||Sato Y. Arai H. Miyata A. Tokita S. Yamamoto K. Tanabe T. Inoue K.|
|Title||Primary structure of alpha-tocopherol transfer protein from rat liver. Homology with cellular retinaldehyde-binding protein.|
|Source||J. Biol. Chem. 268:17705-17710(1993).|
|3||Authors||Sha B. Phillips S.E. Bankaitis V.A. Luo M.|
|Title||Crystal structure of the Saccharomyces cerevisiae phosphatidylinositol-transfer protein.|
|4||Authors||Aravind L. Neuwald A.F. Ponting C.P.|
|Title||Sec14p-like domains in NF1 and Dbl-like proteins indicate lipid regulation of Ras and Rho signaling.|
|Source||Curr. Biol. 9:R195-R197(1999).|
|5||Authors||Zimmer S. Stocker A. Sarbolouki M.N. Spycher S.E. Sassoon J. Azzi A.|
|Title||A novel human tocopherol-associated protein: cloning, in vitro expression, and characterization.|
|Source||J. Biol. Chem. 275:25672-25680(2000).|