PROSITE documentation PDOC50202Major sperm protein (MSP) domain profile
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Nematode sperm are unusual amoeboid cells in which motility is not based on actin, but instead on the major sperm protein (MSP) that polymerizes to form a cytoskeleton constructed from intermeshed filaments. MSP is a dimeric molecule that polymerizes to form non-polar filaments constructed from two helical subfilaments that wind round one another. The filaments then assemble into larger macromolecular assemblies called fibre complexes. MSP is a small (~14 kDa) basic protein typically encoded by a multigene family of up to 28 members [1,2,3,4]. An about 120-amino acid domain similar to MSP has been found in the following proteins:
- Animal Vesicle-Associated Membrane Protein-associated (VAMP-associated) protein family of 33 kDa (VAP33). VAP33 is required for neurotransmitter release. It binds to the v-SNARE synaptobrevin/VAMP which is associated with vesicle fusion. VAP33 has a two-domain structure with its N-terminus being highly homologous to MSP, whereas its C-terminus is based on a putative α-helical coiled-coil combined with an extremly hydrophobic membrane-attachment region [5].
- Nicotiana plumbaginifolia VAP27, a VAP33 homologue. It interacts with the resistance protein Cf9 [6].
- Yeast inositol regulator SCS2, a VAP33 homologue. It is C-terminally anchored to the endoplasmic reticulum [7].
The MSP polypeptide chain has an immunoglobulin-like fold based on a seven-stranded β sandwich measuring approximately 15 A x 20 A x 45 A and having opposing three-stranded and four-stranded β sheets (see <PDB:1MSP>) [1].
The profile we developed covers the entire MSP domain.
Last update:October 2002 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Bullock T.L. Roberts T.M. Stewart M. |
| Title | 2.6 A resolution crystal structure of helices of the motile major sperm protein (MSP) of Caenorhabditis elegans. | |
| Source | J. Mol. Biol. 263:284-296(1996). | |
| PubMed ID | 8913307 |
| 2 | Authors | Baker A.M.E. Roberts T.M. Stewart M. |
| Title | The crystal structure of aminoglycoside-3'-phosphotransferase-IIa, an enzyme responsible for antibiotic resistance. | |
| Source | J. Mol. Biol. 319:491-499(2002). | |
| PubMed ID | 12051923 |
| 3 | Authors | Haaf A. LeClaire L. III Roberts G. Kent H.M. Roberts T.M. Stewart M. Neuhaus D. |
| Title | Solution structure of the motile major sperm protein (MSP) of Ascaris suum - evidence for two manganese binding sites and the possible role of divalent cations in filament formation. | |
| Source | J. Mol. Biol. 284:1611-1624(1998). | |
| PubMed ID | 9878374 |
| 4 | Authors | Smith H.E. Ward S. |
| Title | Identification of protein-protein interactions of the major sperm protein (MSP) of Caenorhabditis elegans. | |
| Source | J. Mol. Biol. 279:605-619(1998). | |
| PubMed ID | 9641981 |
| 5 | Authors | Nishimura Y. Hayashi M. Inada H. Tanaka T. |
| Title | Molecular cloning and characterization of mammalian homologues of vesicle-associated membrane protein-associated (VAMP-associated) proteins. | |
| Source | Biochem. Biophys. Res. Commun. 254:21-26(1999). | |
| PubMed ID | 9920726 |
| 6 | Authors | Laurent F. Labesse G. de Wit P. |
| Title | Molecular cloning and partial characterization of a plant VAP33 homologue with a major sperm protein domain. | |
| Source | Biochem. Biophys. Res. Commun. 270:286-292(2000). | |
| PubMed ID | 10733941 | |
| DOI | 10.1006/bbrc.2000.2387 |
| 7 | Authors | Kagiwada S. Hosaka K. Murata M. Nikawa J.-I. Takatsuki A. |
| Title | The Saccharomyces cerevisiae SCS2 gene product, a homolog of a synaptobrevin-associated protein, is an integral membrane protein of the endoplasmic reticulum and is required for inositol metabolism. | |
| Source | J. Bacteriol. 180:1700-1708(1998). | |
| PubMed ID | 9537365 |
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