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PROSITE documentation PDOC50215

ADAM type metalloprotease domain profile


ADAMs are transmembrane proteins containing both a disintegrin (see <PDOC00351>) and a metalloprotease domain [1]. About two third of the proteins with an ADAM type metalloprotease domain also contain the zinc protease pattern (see <PDOC00129>) which locates the active site of these proteases. As they contain an adhesion domain and a protease domain, ADAM proteins potentially have both cell adhesion and protease activities. They play a role in various biological processes, including fertilization, neurogenesis, myogenesis, embryonic TGF-α release and inflammatory response [2].

ADAMTS proteins [3] form a closely related family of proteases. In these proteins, the metalloprotease and disintegrin domains are flanked by thrombospondin type I (TSP1) repeat (see <PDOC50092>). ADAMTS proteins are soluble, extracellular matrix proteases those known substrates are other extracellular matrix proteins [2].

ADAM proteins also share the metalloprotease and disintegrin domains with the disintegrin class of peptides that are present in snake venom [2,4]. Together, they form the adamlysin/reprolysin subfamily of the metzincin superfamily of Zn-dependent metalloproteinases [4].

Some proteins known to contain an ADAM metalloprotease domain are listed below:

  • Human ADAM 1 and 2 (fertilin α and β). Fertilins are sperm surface membrane proteins that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. They lack proteolytic activity.
  • Human ADAM 8. It may be involved in immune function.
  • Drosophila Kuzbanian. It is involved in neurogenesis. Kuzbanian is a sheddase that has been found to release a soluble form of Delta, a Notch ligand.
  • Vertebrate ADAM 10, the homologue of Kuzbanian.
  • Caenorhabditis elegans Sup-17, the homologue of Kuzbanian. It is involved in Lin-12/NOTCH signaling.
  • Human ADAM 12 (meltrin α). It may be involved in myogenesis.
  • Several snake venom metalloproteinases of the disintegrin family. They are not transmembrane proteins.
  • Mammalian ADAMTS-1. Protease those expression is associated with acute inflammation as well as development of cancer cachexia.
Last update:

December 2001 / First entry.

Technical section

PROSITE method (with tools and information) covered by this documentation:

ADAM_MEPRO, PS50215; ADAM type metalloprotease domain profile  (MATRIX)


1AuthorsWolfsberg T.G., Straight P.D., Gerena R.L., Huovila A.P., Primakoff P., Myles D.G., White J.M.
TitleADAM, a widely distributed and developmentally regulated gene family encoding membrane proteins with a disintegrin and metalloprotease domain.
SourceDev. Biol. 169:378-383(1995).
PubMed ID7750654

2AuthorsPrimakoff P., Myles D.G.
TitleThe ADAM gene family: surface proteins with adhesion and protease activity.
SourceTrends Genet. 16:83-87(2000).
PubMed ID10652535

3AuthorsTang B.L., Hong W.
TitleADAMTS: a novel family of proteases with an ADAM protease domain and thrombospondin 1 repeats.
SourceFEBS Lett. 445:223-225(1999).
PubMed ID10094461

4AuthorsBlack R.A., White J.M.
TitleADAMs: focus on the protease domain.
SourceCurr. Opin. Cell Biol. 10:654-659(1998).
PubMed ID9818177

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