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PROSITE documentation PDOC50531 |
Autonomous mobile genetic elements such as transposons or insertion sequences (IS) encode an enzyme, called transposase, required for excising and inserting the mobile element. The IS21 is among the largest of bacterial IS elements and encodes a transposase designated as istA (ORF1) as well as a second protein istB (ORF2) and represents the IS21/IS408/IS1162 family [E1,1]. The transposases of IS21-like IS contain a helix-turn-helix (HTH) motif in the N-terminal part that probably binds to DNA. An integrase catalytic domain (see <PDOC50994>) forms the central part of these IS21/IS408/IS1162 family transposases.
On the basis of sequence similarities, two types of HTH-domains can be found in IS21-like transposases.
The first, IS21 transposase-type HTH domain, occurs in transposases from the following elements:
The second is a IS408/IS1162 transposase-type HTH domain. Some proteins known to contain this domain:
The profiles we developed cover the entire IS21 transposase-type and IS408/IS1162 transposase-type HTH domains.
Last update:December 2006 / First entry.
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PROSITE methods (with tools and information) covered by this documentation:
1 | Authors | Berger B. Haas D. |
Title | Transposase and cointegrase: specialized transposition proteins of the bacterial insertion sequence IS21 and related elements. | |
Source | Cell. Mol. Life Sci. 58:403-419(2001). | |
PubMed ID | 11315188 |
E1 | Title | https://www-is.biotoul.fr/scripts/ficheIS.php?name=IS21 |