PROSITE documentation PDOC50870Arfaptin homology (AH) domain profile
The arfaptin homology domain (AH) is an about 210-residue region of homology, which has been identified between PICK1 and arfaptins [1]. The AH domain can be found alone or in association with other domains such as PDZ (see <PDOC50106>) [1]. The AH domain of arfaptin has been shown to dimerize and to bind Arf and Rho family GTPases [2,3].
The resolution of arfaptin has shown that the AH domain consists of three α-helices arranged as an extended antiparallel α-helical bundle. Two arfaptin AH domains associate to form a highly elongated, crescent-shaped dimer (see <PDB:1I49>) [2,3].
Some proteins known to contain an AH domain are listed below:
- Animal protein kinase C-binding protein PICK1.
- Animal arfaptin 1 and 2. Arfaptins are effectors of two G proteins, Rac and Arf (ADP-ribosylation factor).
- Mammalian 69 kDa islet cell autoantigen (ICA69) [4]. It is likely to play a role in membrane trafficking at the Golgi complex and immature secretory granules in neurosecretory cells.
The profile we developed covers the entire AH domain.
Last update:November 2003 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Takeya R. Takeshige K. Sumimoto H. |
| Title | Interaction of the PDZ domain of human PICK1 with class I ADP-ribosylation factors. | |
| Source | Biochem. Biophys. Res. Commun. 267:149-155(2000). | |
| PubMed ID | 10623590 | |
| DOI | 10.1006/bbrc.1999.1932 |
| 2 | Authors | Tarricone C. Xiao B. Justin N. Walker P.A. Rittinger K. Gamblin S.J. Smerdon S.J. |
| Title | The structural basis of Arfaptin-mediated cross-talk between Rac and Arf signalling pathways. | |
| Source | Nature 411:215-219(2001). | |
| PubMed ID | 11346801 | |
| DOI | 10.1038/35075620 |
| 3 | Authors | Cherfils J. |
| Title | Structural mimicry of DH domains by Arfaptin suggests a model for the recognition of Rac-GDP by its guanine nucleotide exchange factors. | |
| Source | FEBS Lett. 507:280-284(2001). | |
| PubMed ID | 11696355 |
| 4 | Authors | Spitzenberger F. Pietropaolo S. Verkade P. Habermann B. Lacas-Gervais S. Mziaut H. Pietropaolo M. Solimena M. |
| Title | Islet cell autoantigen of 69 kDa is an arfaptin-related protein associated with the Golgi complex of insulinoma INS-1 cells. | |
| Source | J. Biol. Chem. 278:26166-26173(2003). | |
| PubMed ID | 12682071 | |
| DOI | 10.1074/jbc.M213222200 |
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