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PROSITE documentation PDOC50890PUA domain profile
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PURL: https://purl.expasy.org/prosite/documentation/PDOC50890
The PUA domain, which has been named after PseudoUridine synthase and Archaeosine-specific transglycosylase, is a small, compact domain that consists of 78-83 amino acid residues. The PUA domain is widely conserved in eukaryotic and archaeal RNA modification enzymes and is found in:
- Archaeal and eukaryotic pseudouridine synthases.
- Archaeosine tRNA-guanine transglycosylases.
- A family of predicted ATPases that may be involved in RNA modification.
- A family of predicted archaeal and bacterial rRNA methylases.
- A conserved family of putative novel eukaryotic translation factors.
- Glutamate 5-kinases (EC 2.7.2.11).
The PUA domain can be found alone or in association with other domains, such as the glutamate kinase domain (see <PDOC00701>), the pseudouridine synthase catalytic domain, the phosphoadenosine phosphosulfate (PAPS) reductase domain, the methylase domain, the TGT domain, or the SUI1 domain (see <PDOC00862 >). The PUA domain is supposed to bind to RNA molecules with complex folded structures [1].
The PUA domain contains highly conserved motifs that center around stretches of hydrophobic residues. The domain also contains two highly conserved positions occupied by small amino acids (primarily glycines) [1]. The resolution of the crystal structure of archaeosine tRNA-guanine transglycosylase from Pyrococcus horikoshii has shown that the PUA domain consists of two α-helices and six β-strands, and folds into a β-sandwich structure similar to the oligonucleotide-binding (OB)-fold (see <PDB:1IQ8>) [2].
The profile we developed covers the entire PUA domain.
Last update:November 2002 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Aravind L. Koonin E.V. |
| Title | Novel predicted RNA-binding domains associated with the translation machinery. | |
| Source | J. Mol. Evol. 48:291-302(1999). | |
| PubMed ID | 10093218 |
| 2 | Authors | Ishitani R. Nureki O. Fukai S. Kijimoto T. Nameki N. Watanabe M. Kondo H. Sekine M. Okada N. Nishimura S. Yokoyama S. |
| Title | Crystal structure of archaeosine tRNA-guanine transglycosylase. | |
| Source | J. Mol. Biol. 318:665-677(2002). | |
| PubMed ID | 12054814 | |
| DOI | 10.1016/S0022-2836(02)00090-6 |
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