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PROSITE documentation PDOC50940Chitin-binding type-2 domain profile
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PURL: https://purl.expasy.org/prosite/documentation/PDOC50940
The species distribution of the chitin-binding type-2 domain is complementary to the chitin-binding type-1 domain (see <PDOC00025>). The type-2 is mainly found in animal and baculovirus proteins while the type-1 is found in plant and fungi. Type-2 is characterized by having a 6-cysteine motif: C-x(13,20)-C-x(5,6)-C-x(9,19)-C-x(10,14)-C-x(4,14)-C and several aromatic residues [1].
The structure of the chitin-binding domain of tachycitin has been solved (see <PDB:1DQC>) [2]. It revealed that tachycitin shares a remarkable structural similarity with the chitin-binding type-1 domain of hevein. The overall structure forms a distorted β-sandwich constructed by three-stranded and two-stranded β-sheets connected through a bending loop (Cys-40-Leu-44). The precise chitin binding site (Cys-40 to Gly-60) is perfectly superposable with the chitin binding site of hevein. Furthermore a disulfide bridge connecting Cys-40 and Cys-53 is also present at the same location in the hevein structure [2].
Some protein known to contain a chitin-binding type-2 domain are listed below:
- Animal class-II chitinase. It degrades chitin and chitotriose. In mammals it may participate in the defense against nematodes and other pathogens [3].
- Tachycitin, an antimicrobial protein of horseshoe crab hemocytes.
- Peritrophin-A, a component of peritrophic matrices (acellular linings in the gut of most insects that comprise proteins and sugar polymers, including chitin).
- Drosophila Tequila protein, a serine protease involved in immune response.
- Drosophila Hemolectin, a sugar binding protein involved in defense response.
The profile we developed covers the whole domain.
Last update:July 2016 / Profile revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Gaines P.J. Walmsley S.J. Wisnewski N. |
| Title | Cloning and characterization of five cDNAs encoding peritrophin-A domains from the cat flea, Ctenocephalides felis. | |
| Source | Insect Biochem. Mol. Biol. 33:1061-1073(2003). | |
| PubMed ID | 14563358 |
| 2 | Authors | Suetake T. Tsuda S. Kawabata S. Miura K. Iwanaga S. Hikichi K. Nitta K. Kawano K. |
| Title | Chitin-binding proteins in invertebrates and plants comprise a common chitin-binding structural motif. | |
| Source | J. Biol. Chem. 275:17929-17932(2000). | |
| PubMed ID | 10770921 | |
| DOI | 10.1074/jbc.C000184200; |
| 3 | Authors | Tjoelker L.W. Gosting L. Frey S. Hunter C.L. Trong H.L. Steiner B. Brammer H. Gray P.W. |
| Title | Structural and functional definition of the human chitinase chitin-binding domain. | |
| Source | J. Biol. Chem. 275:514-520(2000). | |
| PubMed ID | 10617646 |
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