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PROSITE documentation PDOC50945 |
The I/LWEQ domain is a ~250-residue actin-binding module that is found in the C-termini of functionally diverse proteins from yeast to mammals. The I/LWEQ domain contains four conserved blocks and has been named after the conserved initial residues of blocks 1-4. The I/LWEQ domain is generally found near the C-terminus and in association with other domains, such as FERM (see <PDOC00566>) or ENTH (see <PDOC50942>). The I/LWEQ domain has been shown to bind to F-actin and to bundle actin filaments [1,2,3].
The I/LWEQ domain contains a proteolysis resistant-core that has an all-α-helical structure composed of five long down-up-down-up-down antiparallel helices connected by short loops and one short connecting α helix arranged in a progressive topology (see <PDB:1R0D>). The proteolysis-resistant core is followed by a C-terminal latch region that adopts an α-helical conformation. The latch region can mediate oligomerization of I/LWEQ domains, possibly as dimers, and this seems to enhance the F-actin-binding ability of the proteolysis-resistant core [3].
Some proteins known to contain a I/LWEQ domain are listed below:
The profile we developed covers the entire I/LWEQ domain.
Note:The I/LWEQ domain has also been called the talin-HIP1/R/Slap2p actin- tethering C-terminal homology (THATCH) domain (HIP1/R/Slap2p denotes similarity of Slap2p to HIP1 and HIP1R) [3].
Last update:February 2006 / Profile and text revised.
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PROSITE method (with tools and information) covered by this documentation:
1 | Authors | McCann R.O. Craig S.W. |
Title | The I/LWEQ module: a conserved sequence that signifies F-actin binding in functionally diverse proteins from yeast to mammals. | |
Source | Proc. Natl. Acad. Sci. U.S.A. 94:5679-5684(1997). | |
PubMed ID | 9159132 |
2 | Authors | McCann R.O. Craig S.W. |
Title | Functional genomic analysis reveals the utility of the I/LWEQ module as a predictor of protein:actin interaction. | |
Source | Biochem. Biophys. Res. Commun. 266:135-140(1999). | |
PubMed ID | 10581178 | |
DOI | 10.1006/bbrc.1999.1776 |
3 | Authors | Brett T.J. Legendre-Guillemin V. McPherson P.S. Fremont D.H. |
Title | Structural definition of the F-actin-binding THATCH domain from HIP1R. | |
Source | Nat. Struct. Mol. Biol. 13:121-130(2006). | |
PubMed ID | 16415883 | |
DOI | 10.1038/nsmb1043 |