Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC51072Mu homology domain (MHD) profile
View entry in original PROSITE document format
View entry in raw text format (no links)
PURL: https://purl.expasy.org/prosite/documentation/PDOC51072
The mu homology domain (MHD) is an ~280 residue protein-protein interaction module, which is found in endocytotic proteins involved in clathrin-mediated endocytosis [1,2,3,4]:
- Mu subunits of adaptor protein (AP) complexes, AP-1, AP-2, AP-3, and AP-4 (see <PDOC00761>).
- Proteins of the stonin family.
- Proteins of the muniscin family: Syp1, FCHO1/2 and SGIP1.
The MHD domain has an elongated, banana-shaped, all β-sheet structure (see <PDB:1BW8>). It can be considered as two β-sandwich subdomains (A and B), with subdomain B inserted between strands 6 and 15 of subdomain A, and joined edge to edge such that the convex surface is a continuous nine-stranded mixed β-sheet that runs the whole length of the molecule. The tyrosine based signal binds to a site on the surface of two parallel β-sheet strands (β1 and β16) in subdomain A [4,5].
The profile we developed covers the entire MHD domain.
Last update:October 2013 / Text revised.
-------------------------------------------------------------------------------
PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Martina J.A. Bonangelino C.J. Aguilar R.C. Bonifacino J.S. |
| Title | Stonin 2: an adaptor-like protein that interacts with components of the endocytic machinery. | |
| Source | J. Cell Biol. 153:1111-1120(2001). | |
| PubMed ID | 11381094 |
| 2 | Authors | Walther K. Krauss M. Diril M.K. Lemke S. Ricotta D. Honing S. Kaiser S. Haucke V. |
| Title | Human stoned B interacts with AP-2 and synaptotagmin and facilitates clathrin-coated vesicle uncoating. | |
| Source | EMBO Rep. 2:634-640(2001). | |
| PubMed ID | 11454741 | |
| DOI | 10.1093/embo-reports/kve134 |
| 3 | Authors | Walther K. Diril M.K. Jung N. Haucke V. |
| Title | Functional dissection of the interactions of stonin 2 with the adaptor complex AP-2 and synaptotagmin. | |
| Source | Proc. Natl. Acad. Sci. U.S.A. 101:964-969(2004). | |
| PubMed ID | 14726597 | |
| DOI | 10.1073/pnas.0307862100 |
| 4 | Authors | Reider A. Barker S.L. Mishra S.K. Im Y.J. Maldonado-Baez L. Hurley J.H. Traub L.M. Wendland B. |
| Title | Syp1 is a conserved endocytic adaptor that contains domains involved in cargo selection and membrane tubulation. | |
| Source | EMBO J. 28:3103-3116(2009). | |
| PubMed ID | 19713939 | |
| DOI | 10.1038/emboj.2009.248 |
| 5 | Authors | Owen D.J. Evans P.R. |
| Title | A structural explanation for the recognition of tyrosine-based endocytotic signals. | |
| Source | Science 282:1327-1332(1998). | |
| PubMed ID | 9812899 |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.