PROSITE documentation PDOC51114
F-box-associated (FBA) domain profile


F-box proteins have a bipartite structure: they contain a carboxy-terminal domain that interacts with substrates and a 42-48 amino-acid F-box domain (see <PDOC50181>) which binds to the protein Skp1. A subset of F-box proteins is characterized by a ~180-residue carboxy-terminal region, which has been called the F-box-associated (FBA) domain [1,2]. A FBA domain has also been identified in the catfish, tilapia, and zebrafish nonspecific cytotoxic cell receptor proteins (NCCRP-1), which do not contain the F-box domain. NCCRP-1 may function as an antigen recognition molecule and, as such, may participate in innate immunity in teleosts [2,3]. The FBA domain is likely to be a glycoprotein-binding module [4,5].

The FBA domain is an ellipsoid composed of a ten-stranded antiparallel β-sandwich with two α-helices (see <PDB:1UMH>) [5].

Some proteins known to contain a FBA domain are listed below:

  • Mammalian F-box only protein 2.
  • Mammalian F-box only protein 6.
  • Mammalian F-box only protein 17.
  • Mammalian F-box only protein 27.
  • Mammalian F-box only protein 44.
  • Teleostian NCCRP-1.
  • Caenorhabditis elegans hypothetical protein C14B1.3.

The profile we developed covers the entire FBA domain.

Last update:

July 2005 / First entry.


Technical section

PROSITE method (with tools and information) covered by this documentation:

FBA, PS51114; F-box-associated (FBA) domain profile  (MATRIX)


1AuthorsWinston J.T. Koepp D.M. Zhu C. Elledge S.J. Harper J.W.
TitleA family of mammalian F-box proteins.
SourceCurr. Biol. 9:1180-1182(1999).
PubMed ID10531037

2AuthorsIlyin G.P. Serandour A.-L. Pigeon C. Rialland M. Glaise D. Guguen-Guillouzo C.
TitleA new subfamily of structurally related human F-box proteins.
SourceGene 296:11-20(2002).
PubMed ID12383498

3AuthorsJaso-Friedmann L. Peterson D.S. Gonzalez D.S. Evans D.L.
TitleThe antigen receptor (NCCRP-1) on catfish and zebrafish nonspecific cytotoxic cells belongs to a new gene family characterized by an F-box-associated domain.
SourceJ. Mol. Evol. 54:386-395(2002).
PubMed ID11847564

4AuthorsYoshida Y. Tokunaga F. Chiba T. Iwai K. Tanaka K. Tai T.
TitleFbs2 is a new member of the E3 ubiquitin ligase family that recognizes sugar chains.
SourceJ. Biol. Chem. 278:43877-43884(2003).
PubMed ID12939278

5AuthorsMizushima T. Hirao T. Yoshida Y. Lee S.J. Chiba T. Iwai K. Yamaguchi Y. Kato K. Tsukihara T. Tanaka K.
TitleStructural basis of sugar-recognizing ubiquitin ligase.
SourceNat. Struct. Mol. Biol. 11:365-370(2004).
PubMed ID14990996

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