|PROSITE documentation PDOC51170|
The cell wall-binding repeat (CW) is a ~20 amino acid residue module essentially found in two bacterial Gram-positive protein families: choline binding proteins and glucosyltransferases (EC 22.214.171.124). In choline-binding proteins cell wall binding repeats bind to choline moieties of both teichoic and lipoteichoic acids, two components peculiar to the cell surface of Gram-positive bacteria [1,2]. In glucosyltransferases the region spanning the CW repeats is a glucan binding domain .
Several crystal structures of CW have been solved [4,5]. In the choline binding protein LytA (see <PDB:1HCX>), the repeats adopt a solenoid fold consisting exclusively of β-hairpins that stack to form a left-handed superhelix with a boomerang-like shape. The choline groups bind between β-hairpin 'steps' of the superhelix . In Cpl-1 CW repeats assemble in two sub-domains: an N-terminal superhelical moiety similar to the LytA one and a C-terminal β-sheet involved in interactions with the lysozyme domain. Choline is bound between repeats 1 and 2, and, 2 and 3 of the superhelical sub-domain .
Some proteins known to contain cell-wall binding repeats:
The profile we developed covers the 20 amino acids of the CW repeat.Note:
The cell wall binding repeats are also known as the choline-binding repeats (ChBr) or the choline-binding domain (ChBD).Last update:
December 2005 / First entry.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Garcia-Bustos J.F. Tomasz A.|
|Title||Teichoic acid-containing muropeptides from Streptococcus pneumoniae as substrates for the pneumococcal autolysin.|
|Source||J. Bacteriol. 169:447-453(1987).|
|2||Authors||Lopez R. Garcia E.|
|Title||Recent trends on the molecular biology of pneumococcal capsules, lytic enzymes, and bacteriophage.|
|Source||FEMS. Microbiol. Rev. 28:553-580(2004).|
|3||Authors||Shah D.S. Joucla G. Remaud-Simeon M. Russell R.R.|
|Title||Conserved repeat motifs and glucan binding by glucansucrases of oral streptococci and Leuconostoc mesenteroides.|
|Source||J. Bacteriol. 186:8301-8308(2004).|
|4||Authors||Fernandez-Tornero C. Lopez R. Garcia E. Gimenez-Gallego G. Romero A.|
|Title||A novel solenoid fold in the cell wall anchoring domain of the pneumococcal virulence factor LytA.|
|Source||Nat. Struct. Biol. 8:1020-1024(2001).|
|5||Authors||Hermoso J.A. Monterroso B. Albert A. Galan B. Ahrazem O. Garcia P. Martinez-Ripoll M. Garcia J.L. Menendez M.|
|Title||Structural basis for selective recognition of pneumococcal cell wall by modular endolysin from phage Cp-1.|