|PROSITE documentation PDOC51214|
Cytosolic proteins bearing a classical nuclear localization signal (NLS) (see <PDOC00015>) enter the nucleus bound to a heterodimer of importin-α and importin-β (also called karyopherin-α and -β). Importin-α contains the NLS-binding site and importin-β is responsible for the docking of the importin-substrate complex to the filaments of the nuclear pore complex (NPC) and its translocation through the pore. Importin-α consists of two functional domains, a highly basic amino-terminal region of roughly 40 amino-acid residues (the IBB domain) responsible for importin-β binding, and an NLS-binding domain built of armadillo (ARM) repeats (see <PDOC50176>). Appart from the classical protein-import pathway, importin-β is also involved in the nuclear import of spliceosomal small nuclear ribonucleoprotein particles (snRNPs), which is mediated by the 45K protein snurportin-1. Snurportin-1 is functionally analogous to importin-α, as it recognizes the trimethylguanosine (m3G) cap structure of the small nuclear RNAs U1, U2, U4 and U5, and binds to importin-β through an IBB-domain. Snurportin-1 contains an N-terminal IBB domain and a C-terminal m3G-cap-binding region with no structural similarity to the arm repeat domain of importin-α [1,2,3].
The IBB domain is an L-shapped molecule with an N-terminal extended moiety and a C-terminal helix running in mutually perpendicular directions (see <PDB:1QGK>). The IBB domain is intimately bound on the inner surface of importin-β that contains many acidic residues and is thus complementary to the highly positively charged IBB domain [1,2].
The profile we developed covers the entire IBB domain.Last update:
June 2006 / First entry.
PROSITE method (with tools and information) covered by this documentation:
|Title||Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin alpha.|
|Source||Nat. Struct. Biol. 6:388-397(1999).|
|2||Authors||Cingolani G. Petosa C. Weis K. Mueller C.W.|
|Title||Structure of importin-beta bound to the IBB domain of importin-alpha.|
|3||Authors||Huber J. Cronshagen U. Kadokura M. Marshallsay C. Wada T. Sekine M. Luehrmann R.|
|Title||Snurportin1, an m3G-cap-specific nuclear import receptor with a novel domain structure.|
|Source||EMBO J. 17:4114-4126(1998).|