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PROSITE documentation PDOC51216 |
The nebulin-like motif or nebulin repeat is a tandemly repeated actin-binding module of about 35 amino acids. The repeat is named after the nebulin protein, which is a large protein specific for vertebrate skeletal muscle that may regulate the length of thin filaments. Nebulin contains about 185 copies of the repeat and those in the central part of nebulin are organized into seven-module super-repeats, which seems to reflect an interaction with tropomysin/troponin [1]. Nebulin repeats occur in metazoan actin-binding proteins, most of which are specific for muscle tissues. Most nebulin repeat proteins contain a C-terminal SH3 domain (see <PDOC50002>) and/or a N-terminal LIM zinc-binding domain (see <PDOC00382>). The repeats in nebulin and nebulette bind filamentous actin (F-actin) and may also associate with tropomyosin and troponin. The nebulin repeat has a predicted α-helical secondary structure and contains a central conserved SXXXY motif [1,2,3].
Some proteins known to contain nebulin repeats:
The profile we developed covers the entire nebulin repeat.
Last update:June 2006 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
1 | Authors | Pfuhl M. Winder S.J. Pastore A. |
Title | Nebulin, a helical actin binding protein. | |
Source | EMBO J. 13:1782-1789(1994). | |
PubMed ID | 8168478 |
2 | Authors | Millevoi S. Trombitas K. Kolmerer B. Kostin S. Schaper J. Pelin K. Granzier H. Labeit S. |
Title | Characterization of nebulette and nebulin and emerging concepts of their roles for vertebrate Z-discs. | |
Source | J. Mol. Biol. 282:111-123(1998). | |
PubMed ID | 9733644 | |
DOI | 10.1006/jmbi.1998.1999 |
3 | Authors | Ogut O. Hossain M.M. Jin J.P. |
Title | Interactions between nebulin-like motifs and thin filament regulatory proteins. | |
Source | J. Biol. Chem. 278:3089-3097(2003). | |
PubMed ID | 12446728 | |
DOI | 10.1074/jbc.M205853200 |