PROSITE documentation PDOC51253CENPB-type HTH domain profile
The CENPB-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 70-75 amino acids, present in eukaryotic centromere proteins and transposases. The domain is named after the mammalian major centromere autoantigen B or centromere protein B (CENP-B), which is a fundamental centromere component of chromosomes. The N-terminus of CENP-B contains two DNA-binding HTH domains, which bind to adjacent major grooves of DNA. The N-terminus of CENP-B is formed by a psq-type HTH domain (see <PDOC50960>) and C-terminal to this domain lies the CENPB-type HTH domain. These two HTH domains together bind specifically to a 17-base-pair sequence, the CENP-B box, which occurs in α-satellite DNA in human centromeres [1].
The structure of the CENPB-type HTH domain is composed of three α-helices (see <PDB:1HLV>). The second and third helices connected via a turn comprise the helix-turn-helix motif. Helix 3 is termed the recognition helix as it binds the DNA major groove, like in other HTHs. In CENP-B this domain recognizes site 3 of the CENP-B box, while the preceding psq-type HTH (see <PDOC50960>) binds site 1 of the CENP-B box, and a connecting linker loop binds in the minor groove of DNA and recognizes site 2 [2].
Some proteins known to contain a CENPB-type HTH domain:
- Mammalian centromere protein B (CENP-B), associated with the centromere and specifically binding DNA to the CENP-B box.
- Mammalian jerky protein, involved in epileptic seizures in mice.
- Mammalian Pogo transposases.
- Fission yeast ARS-binding protein 1 (abp1) and CENP-B homolog proteins (CBHP-1 and 2), which are centromere proteins.
- Candida albicans protein PDC2.
- Fungal transposases.
The profile we developed covers the entire CENPB-type HTH domain, including the N-terminal linker loop.
Last update:September 2006 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Tanaka Y. Tachiwana H. Yoda K. Masumoto H. Okazaki T. Kurumizaka H. Yokoyama S. |
| Title | Human centromere protein B induces translational positioning of nucleosomes on alpha-satellite sequences. | |
| Source | J. Biol. Chem. 280:41609-41618(2005). | |
| PubMed ID | 16183641 | |
| DOI | 10.1074/jbc.M509666200 |
| 2 | Authors | Tanaka Y. Nureki O. Kurumizaka H. Fukai S. Kawaguchi S. Ikuta M. Iwahara J. Okazaki T. Yokoyama S. |
| Title | Crystal structure of the CENP-B protein-DNA complex: the DNA-binding domains of CENP-B induce kinks in the CENP-B box DNA. | |
| Source | EMBO J. 20:6612-6618(2001). | |
| PubMed ID | 11726497 | |
| DOI | 10.1093/emboj/20.23.6612 |
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