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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
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Amos Bairoch

PROSITE documentation PDOC51329
C-CAP/cofactor C-like domain profile


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PURL: https://purl.expasy.org/prosite/documentation/PDOC51329

Description

The C-CAP/cofactor C-like domain is present in several cytoskeleton-related proteins, which also contain a number of additional domains [1,2,3,4]:

  • Eukaryotic cyclase-associated protein (CAP or SRV2) (see <PDOC00835>), a modular actin monomer binding that directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
  • Vertebrate retinitis pigmentosa 2 (XRP2). In human, the protein responsible for X-linked forms of retinitis pigmentosa, a disease characterized by severe retinal degeneration.
  • Eukaryotic tubulin-specific chaperone cofactor C (TBCC), a GTPase- activating component of the tubulin-folding supercomplex, which directs the assembly of the α- and β-tubulin heterodimer.

The cyclase-associated protein C-CAP/cofactor C-like domain binds G-actin and is responsible for oligomerization of the entire CAP molecule [1], whereas the XRP2 C-CAP/cofactor C-like domain is required for binding of ADP ribosylation factor-like protein 3 (Arl3) [2].

The central core of the C-CAP/cofactor C-like domain is composed of six coils of right-handed parallel β-helix, termed coils 1-6, which form an elliptical barrel with a tightly packed interior. Each β-helical coil is composed of three relatively short β-strands, designated a-c, separated by sharp turns. Flanking the central β-helical core is an N-terminal β-strand, β0, that packs antiparallel to the core, and strand β7 packs antiparallel to the core near the C-terminal end of the parallel β-helix (see <PDB:1K4Z>) [1,2].

The profile we developed covers the C-CAP/cofactor C-like domain.

Last update:

August 2007 / First entry.

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Technical section

PROSITE method (with tools and information) covered by this documentation:

C_CAP_COFACTOR_C, PS51329; C-CAP/cofactor C-like domain profile  (MATRIX)


References

1AuthorsDodatko T. Fedorov A.A. Grynberg M. Patskovsky Y. Rozwarski D.A. Jaroszewski L. Aronoff-Spencer E. Kondraskina E. Irving T. Godzik A. Almo S.C.
TitleCrystal structure of the actin binding domain of the cyclase-associated protein.
SourceBiochemistry 43:10628-10641(2004).
PubMed ID15311924
DOI10.1021/bi049071r

2AuthorsKuehnel K. Veltel S. Schlichting I. Wittinghofer A.
TitleCrystal structure of the human retinitis pigmentosa 2 protein and its interaction with Arl3.
SourceStructure 14:367-378(2006).
PubMed ID16472755
DOI10.1016/j.str.2005.11.008

3AuthorsStephan A. Vaughan S. Shaw M.K. Gull K. McKean P.G.
TitleAn Essential Quality Control Mechanism at the Eukaryotic Basal Body Prior to Intraflagellar Transport.
SourceTraffic 0:0-0(2007).
PubMed ID17645436
DOI10.1111/j.1600-0854.2007.00611.x

4AuthorsGrynberg M. Jaroszewski L. Godzik A.
TitleDomain analysis of the tubulin cofactor system: a model for tubulin folding and dimerization.
SourceBMC Bioinformatics 4:46-46(2003).
PubMed ID14536023
DOI10.1186/1471-2105-4-46



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