PROSITE documentation PDOC51351TFIIE beta central core DNA-binding domain profile
Initiation of eukaryotic mRNA transcription requires melting of promoter DNA with the help of the general transcription factors TFIIE and TFIIH. TFIIE consists of a large and a small subunit, referred to as α (see <PDOC51344>) and β, respectively, in higher eukaryotes. TFIIE β has been found to bind to the region where the promoter starts to open to be single-stranded upon transcription initiation by RNA polymerase II. The ~120-residue central core domain of TFIIE β plays a role in double-stranded DNA binding of TFIIE [1].
The TFIIE β central core DNA-binding domain consists of three helices with a β hairpin at the C-terminus, resembling the winged helix proteins (see <PDB:1D8J>). It shows a novel double-stranded DNA-binding activity where the DNA-binding surface locates on the opposite side to the previously reported winged helix motif by forming a positively charged furrow [1].
The profile we developed covers the entire TFIIE β central core DNA-binding domain.
Last update:December 2007 / First entry.
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| 1 | Authors | Okuda M. Watanabe Y. Okamura H. Hanaoka F. Ohkuma Y. Nishimura Y. |
| Title | Structure of the central core domain of TFIIEbeta with a novel double-stranded DNA-binding surface. | |
| Source | EMBO J. 19:1346-1356(2000). | |
| PubMed ID | 10716934 | |
| DOI | 10.1093/emboj/19.6.1346 |
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