Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC51351TFIIE beta central core DNA-binding domain profile
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PURL: https://purl.expasy.org/prosite/documentation/PDOC51351
Initiation of eukaryotic mRNA transcription requires melting of promoter DNA with the help of the general transcription factors TFIIE and TFIIH. TFIIE consists of a large and a small subunit, referred to as α (see <PDOC51344>) and β, respectively, in higher eukaryotes. TFIIE β has been found to bind to the region where the promoter starts to open to be single-stranded upon transcription initiation by RNA polymerase II. The ~120-residue central core domain of TFIIE β plays a role in double-stranded DNA binding of TFIIE [1].
The TFIIE β central core DNA-binding domain consists of three helices with a β hairpin at the C-terminus, resembling the winged helix proteins (see <PDB:1D8J>). It shows a novel double-stranded DNA-binding activity where the DNA-binding surface locates on the opposite side to the previously reported winged helix motif by forming a positively charged furrow [1].
The profile we developed covers the entire TFIIE β central core DNA-binding domain.
Last update:December 2007 / First entry.
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| 1 | Authors | Okuda M. Watanabe Y. Okamura H. Hanaoka F. Ohkuma Y. Nishimura Y. |
| Title | Structure of the central core domain of TFIIEbeta with a novel double-stranded DNA-binding surface. | |
| Source | EMBO J. 19:1346-1356(2000). | |
| PubMed ID | 10716934 | |
| DOI | 10.1093/emboj/19.6.1346 |
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