PROSITE documentation PDOC51363W2 domain profile
Translation initiation is a sophisticated, well regulated and highly coordinated cellular process in eukaryotes, in which at least 11 eukayrotic initiation factors (eIFs) are included. The W2 domain (two invariant tryptophans) is a region of ~165 amino acids which is found in the C-terminus of the following eIFs [1,2,3,4,5]:
- Eukaryotic translation initiation factor 2B epsilon (eIF-2B-epsilon).
- Eukaryotic translation initiation factor 4 γ (eIF-4-γ).
- Eukaryotic translation initiation factor 5 (eIF-5), a GTPase-activating protein (GAP) specific for eIF2.
The W2 domain has a globular fold and is exclusively composed out of α-helices (see <PDB:2FUL>) [3,4,5]. The structure can be divided into a structural C-terminal core onto which the two N-terminal helices are attached. The core contains two aromatic/acidic residue-rich regions (AA boxes), which are important for mediating protein-protein interactions.
The profile we developed covers the entire W2 domain.
Last update:March 2008 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Koonin E.V. |
| Title | Multidomain organization of eukaryotic guanine nucleotide exchange translation initiation factor eIF-2B subunits revealed by analysis of conserved sequence motifs. | |
| Source | Protein Sci. 4:1608-1617(1995). | |
| PubMed ID | 8520487 |
| 2 | Authors | Aravind L. Koonin E.V. |
| Title | Eukaryote-specific domains in translation initiation factors: implications for translation regulation and evolution of the translation system. | |
| Source | Genome Res. 10:1172-1184(2000). | |
| PubMed ID | 10958635 |
| 3 | Authors | Boesen T. Mohammad S.S. Pavitt G.D. Andersen G.R. |
| Title | Structure of the catalytic fragment of translation initiation factor 2B and identification of a critically important catalytic residue. | |
| Source | J. Biol. Chem. 279:10584-10592(2004). | |
| PubMed ID | 14681227 | |
| DOI | 10.1074/jbc.M311055200 |
| 4 | Authors | Wei Z. Xue Y. Xu H. Gong W. |
| Title | Crystal structure of the C-terminal domain of S.cerevisiae eIF5. | |
| Source | J. Mol. Biol. 359:1-9(2006). | |
| PubMed ID | 16616930 | |
| DOI | 10.1016/j.jmb.2006.03.037 |
| 5 | Authors | Bieniossek C. Schuetz P. Bumann M. Limacher A. Uson I. Baumann U. |
| Title | The crystal structure of the carboxy-terminal domain of human translation initiation factor eIF5. | |
| Source | J. Mol. Biol. 360:457-465(2006). | |
| PubMed ID | 16781736 | |
| DOI | 10.1016/j.jmb.2006.05.021 |
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