PROSITE documentation PDOC51446
Pacifastin domain profile


The pacifastin family is composed of proteinase inhibitors from arthropods:

  • Freshwater crayfish pacifastin light chain.
  • Migratory locust LMPI-1 to -3.
  • Desert locust SGPI-1 to -5.
  • Yellowfever mosquito AAPP-1.
  • African malaria mosquito AGPP-1.

These proteinase inhibitors belong to family I19 [E1] and share a pacifastin domain of ~35 residues, which contains a characteristic pattern of six conserved cysteine residues (C-x(9,12)-C-N-x-C-x-C-x(2,3)-G-x(3,6)-C-T-x(3)-C). The pacifastin domain consists of a twisted β-sheet composed of three antiparallel strands and stabilized by an identical pattern (C1-C4, C2-C6, C3-C5) of disulfide bridges (see <PDB:1GL1>) [1,2,3,4,5,6].

The profile we developed covers the entire pacifastin domain.

Last update:

April 2009 / First entry.


Technical section

PROSITE method (with tools and information) covered by this documentation:

PACIFASTIN, PS51446; Pacifastin domain profile  (MATRIX)


1AuthorsLiang Z. Sottrup-Jensen L. Aspan A. Hall M. Soederhaell K.
TitlePacifastin, a novel 155-kDa heterodimeric proteinase inhibitor containing a unique transferrin chain.
SourceProc. Natl. Acad. Sci. U.S.A. 94:6682-6687(1997).
PubMed ID9192625

2AuthorsRoussel A. Mathieu M. Dobbs A. Luu B. Cambillau C. Kellenberger C.
TitleComplexation of two proteic insect inhibitors to the active site of chymotrypsin suggests decoupled roles for binding and selectivity.
SourceJ. Biol. Chem. 276:38893-38898(2001).
PubMed ID11495915

3AuthorsSimonet G. Claeys I. Vanden Broeck J.
TitleStructural and functional properties of a novel serine protease inhibiting peptide family in arthropods.
SourceComp. Biochem. Physiol. 132B:247-255(2002).
PubMed ID11997226

4AuthorsGaspari Z. Patthy A. Graf L. Perczel A.
TitleComparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria.
SourceEur. J. Biochem. 269:527-537(2002).
PubMed ID11856311

5AuthorsSimonet G. Breugelmans B. Proost P. Claeys I. Van Damme J. De Loof A. Vanden Broeck J.
TitleCharacterization of two novel pacifastin-like peptide precursor isoforms in the desert locust (Schistocerca gregaria): cDNA cloning, functional analysis and real-time RT-PCR gene expression studies.
SourceBiochem. J. 388:281-289(2005).
PubMed ID15631618

6AuthorsLeone P. Roussel A. Kellenberger C.
TitleStructure of Locusta migratoria protease inhibitor 3 (LMPI-3) in complex with Fusarium oxysporum trypsin.
SourceActa Crystallogr. D 64:1165-1171(2008).
PubMed ID19020355


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