PROSITE documentation PDOC51446Pacifastin domain profile
The pacifastin family is composed of proteinase inhibitors from arthropods:
- Freshwater crayfish pacifastin light chain.
- Migratory locust LMPI-1 to -3.
- Desert locust SGPI-1 to -5.
- Yellowfever mosquito AAPP-1.
- African malaria mosquito AGPP-1.
These proteinase inhibitors belong to family I19 [E1] and share a pacifastin domain of ~35 residues, which contains a characteristic pattern of six conserved cysteine residues (C-x(9,12)-C-N-x-C-x-C-x(2,3)-G-x(3,6)-C-T-x(3)-C). The pacifastin domain consists of a twisted β-sheet composed of three antiparallel strands and stabilized by an identical pattern (C1-C4, C2-C6, C3-C5) of disulfide bridges (see <PDB:1GL1>) [1,2,3,4,5,6].
The profile we developed covers the entire pacifastin domain.
Last update:April 2009 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Liang Z. Sottrup-Jensen L. Aspan A. Hall M. Soederhaell K. |
| Title | Pacifastin, a novel 155-kDa heterodimeric proteinase inhibitor containing a unique transferrin chain. | |
| Source | Proc. Natl. Acad. Sci. U.S.A. 94:6682-6687(1997). | |
| PubMed ID | 9192625 |
| 2 | Authors | Roussel A. Mathieu M. Dobbs A. Luu B. Cambillau C. Kellenberger C. |
| Title | Complexation of two proteic insect inhibitors to the active site of chymotrypsin suggests decoupled roles for binding and selectivity. | |
| Source | J. Biol. Chem. 276:38893-38898(2001). | |
| PubMed ID | 11495915 | |
| DOI | 10.1074/jbc.M105707200 |
| 3 | Authors | Simonet G. Claeys I. Vanden Broeck J. |
| Title | Structural and functional properties of a novel serine protease inhibiting peptide family in arthropods. | |
| Source | Comp. Biochem. Physiol. 132B:247-255(2002). | |
| PubMed ID | 11997226 |
| 4 | Authors | Gaspari Z. Patthy A. Graf L. Perczel A. |
| Title | Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria. | |
| Source | Eur. J. Biochem. 269:527-537(2002). | |
| PubMed ID | 11856311 |
| 5 | Authors | Simonet G. Breugelmans B. Proost P. Claeys I. Van Damme J. De Loof A. Vanden Broeck J. |
| Title | Characterization of two novel pacifastin-like peptide precursor isoforms in the desert locust (Schistocerca gregaria): cDNA cloning, functional analysis and real-time RT-PCR gene expression studies. | |
| Source | Biochem. J. 388:281-289(2005). | |
| PubMed ID | 15631618 | |
| DOI | 10.1042/BJ20041414 |
| 6 | Authors | Leone P. Roussel A. Kellenberger C. |
| Title | Structure of Locusta migratoria protease inhibitor 3 (LMPI-3) in complex with Fusarium oxysporum trypsin. | |
| Source | Acta Crystallogr. D 64:1165-1171(2008). | |
| PubMed ID | 19020355 | |
| DOI | 10.1107/S0907444908030400 |
| E1 | Title | https://www.ebi.ac.uk/merops/cgi-bin/merops.cgi?id=I19 |
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