Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC51467HARP domain profile
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PURL: https://purl.expasy.org/prosite/documentation/PDOC51467
SMARCAL1 (SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A-like1), also known as DNA-dependent ATPase A and HARP (Hep-A-related proteins), maintains genome integrity during DNA replication. SMARCAL1 has ATP-dependent annealing helicase activity, which helps to stabilize stalled replication forks and facilitate DNA repair during replication. Biochemically, SMARCAL1 can bind to DNA that contains single- and double-stranded regions such as forks and DNA hairpins. DNA binding activates its ATPase activity, and this activity promotes DNA single-stranded annealing [1,2].
SMARCAL1 is a multifunctional protein. The ATPase domain, which lies in the C-terminal half of the protein, is split into two regions of primary amino acid sequence by a 115-amino-acid linker sequence (see <PDOC51192>). The N-terminal half of the protein contains a highly sequence conserved ssDNA-binding protein replication protein A (RPA)-binding domain, and one or two HARP domain(s). The evolutionarily conserved HARP domain determines the annealing helicase activity required for the in vivo and in vitro functions of SMARCAL1 [1,2].
The profile we developed covers the entire HARP domain.
Last update:February 2012 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Ghosal G. Yuan J. Chen J. |
| Title | The HARP domain dictates the annealing helicase activity of HARP/SMARCAL1. | |
| Source | EMBO Rep. 12:574-580(2011). | |
| PubMed ID | 21525954 | |
| DOI | 10.1038/embor.2011.74 |
| 2 | Authors | Betous R. Mason A.C. Rambo R.P. Bansbach C.E. Badu-Nkansah A. Sirbu B.M. Eichman B.F. Cortez D. |
| Title | SMARCAL1 catalyzes fork regression and Holliday junction migration to maintain genome stability during DNA replication. | |
| Source | Genes Dev. 26:151-162(2012). | |
| PubMed ID | 22279047 | |
| DOI | 10.1101/gad.178459.111 |
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