Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC51522Zinc finger nanos-type profile
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PURL: https://purl.expasy.org/prosite/documentation/PDOC51522
Nanos is a highly conserved RNA-binding protein in higher eukaryotes and functions as a key regulatory protein in translational control using a 3' untranslated region during the development and maintenance of germ cells. Nanos comprises a non-conserved amino-terminus and highly conserved carboxy-terminal regions. The C-terminal region has two conserved Cys-Cys-His-Cys (CCHC)-type zinc-finger motifs that are indispensable for nanos function [1,2,3].
The structure of the nanos-type zinc finger is composed of two independent zinc-finger (ZF) lobes, the N-terminal ZF1 and the C-terminal ZF2, which are connected by a linker helix (see <PDB:3ALR>) [3]. These lobes create a large cleft. Zinc ions in ZF1 and ZF2 are bound to the CCHC motif by tetrahedral coordination.
The profile we developed covers the entire nanos-type zinc finger.
Last update:February 2011 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Mosquera L. Forristall C. Zhou Y. King M.L. |
| Title | A mRNA localized to the vegetal cortex of Xenopus oocytes encodes a protein with a nanos-like zinc finger domain. | |
| Source | Development 117:377-386(1993). | |
| PubMed ID | 8223259 |
| 2 | Authors | Subramaniam K. Seydoux G. |
| Title | nos-1 and nos-2, two genes related to Drosophila nanos, regulate primordial germ cell development and survival in Caenorhabditis elegans. | |
| Source | Development 126:4861-4871(1999). | |
| PubMed ID | 10518502 |
| 3 | Authors | Hashimoto H. Hara K. Hishiki A. Kawaguchi S. Shichijo N. Nakamura K. Unzai S. Tamaru Y. Shimizu T. Sato M. |
| Title | Crystal structure of zinc-finger domain of Nanos and its functional implications. | |
| Source | EMBO. Rep. 11:848-853(2010). | |
| PubMed ID | 20948543 | |
| DOI | 10.1038/embor.2010.155 |
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