PROSITE documentation PDOC51525NET domain profile
The bromodomain and extraterminal (BET) proteins are a class of transcriptional regulators whose members can be found in animals, plants and fungi. BET proteins are involved in diverse cellular phenomena such as meiosis, cell-cycle control, and homeosis and have been suggested to modulate chromatin structure and affect transcription via a sequence-independent mechanism. BET proteins are defined as having one (plants) or two (animals/yeast) bromodomains (see <PDOC00550>) and an Extra Terminal (ET) domain. The ET domain consists of three separate regions, only one of which, the N-terminal ET (NET) domain is conserved in all BET proteins. The function of the NET domain is assumed to be protein binding [1,2,3,4].
The structure of the NET domain comprises three α-helices and a characteristic loop region of an irregular but well-defined structure (see <PDB:2JNS>). The NET structure has an acidic patch that forms a continuous ridge with a hydrophobic cleft. which may interact with other proteins and/or DNA [4].
Some proteins known to contain a NET domain are listed below:
- Human RING3 (now designated Brd2)
- Murine MCAP (now desihnated Brd4)
- Drosophila Fsh
- Yeast Bdf1 and Bdf2.
- Arabidopsis imbibition-inducible (IMB1), plays a role in abscisic acid (ABA) and phytochrome A (phyA) mediated responses of seed germination.
The profile we developed covers the entire NET domain.
Last update:July 2012 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Lygerou Z. Conesa C. Lesage P. Swanson R.N. Ruet A. Carlson M. Sentenac A. Seraphin B. |
| Title | The yeast BDF1 gene encodes a transcription factor involved in the expression of a broad class of genes including snRNAs. | |
| Source | Nucleic Acids Res. 22:5332-5340(1994). | |
| PubMed ID | 7816623 |
| 2 | Authors | Florence B. Faller D.V. |
| Title | You bet-cha: a novel family of transcriptional regulators. | |
| Source | Front. Biosci. 6:D1008-D1018(2001). | |
| PubMed ID | 11487468 |
| 3 | Authors | Duque P. Chua N.-H. |
| Title | IMB1, a bromodomain protein induced during seed imbibition, regulates ABA-and phyA-mediated responses of germination in Arabidopsis. | |
| Source | Plant. J. 35:787-799(2003). | |
| PubMed ID | 12969431 |
| 4 | Authors | Lin Y.-J. Umehara T. Inoue M. Saito K. Kigawa T. Jang M.K. Ozato K. Yokoyama S. Padmanabhan B. Guentert P. |
| Title | Solution structure of the extraterminal domain of the bromodomain-containing protein BRD4. | |
| Source | Protein Sci. 17:2174-2179(2008). | |
| PubMed ID | 18815416 | |
| DOI | 10.1110/ps.037580.108 |
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