PROSITE documentation PDOC51525
NET domain profile


The bromodomain and extraterminal (BET) proteins are a class of transcriptional regulators whose members can be found in animals, plants and fungi. BET proteins are involved in diverse cellular phenomena such as meiosis, cell-cycle control, and homeosis and have been suggested to modulate chromatin structure and affect transcription via a sequence-independent mechanism. BET proteins are defined as having one (plants) or two (animals/yeast) bromodomains (see <PDOC00550>) and an Extra Terminal (ET) domain. The ET domain consists of three separate regions, only one of which, the N-terminal ET (NET) domain is conserved in all BET proteins. The function of the NET domain is assumed to be protein binding [1,2,3,4].

The structure of the NET domain comprises three α-helices and a characteristic loop region of an irregular but well-defined structure (see <PDB:2JNS>). The NET structure has an acidic patch that forms a continuous ridge with a hydrophobic cleft. which may interact with other proteins and/or DNA [4].

Some proteins known to contain a NET domain are listed below:

  • Human RING3 (now designated Brd2)
  • Murine MCAP (now desihnated Brd4)
  • Drosophila Fsh
  • Yeast Bdf1 and Bdf2.
  • Arabidopsis imbibition-inducible (IMB1), plays a role in abscisic acid (ABA) and phytochrome A (phyA) mediated responses of seed germination.

The profile we developed covers the entire NET domain.

Last update:

July 2012 / First entry.


Technical section

PROSITE method (with tools and information) covered by this documentation:

NET, PS51525; NET domain profile  (MATRIX)


1AuthorsLygerou Z. Conesa C. Lesage P. Swanson R.N. Ruet A. Carlson M. Sentenac A. Seraphin B.
TitleThe yeast BDF1 gene encodes a transcription factor involved in the expression of a broad class of genes including snRNAs.
SourceNucleic Acids Res. 22:5332-5340(1994).
PubMed ID7816623

2AuthorsFlorence B. Faller D.V.
TitleYou bet-cha: a novel family of transcriptional regulators.
SourceFront. Biosci. 6:D1008-D1018(2001).
PubMed ID11487468

3AuthorsDuque P. Chua N.-H.
TitleIMB1, a bromodomain protein induced during seed imbibition, regulates ABA-and phyA-mediated responses of germination in Arabidopsis.
SourcePlant. J. 35:787-799(2003).
PubMed ID12969431

4AuthorsLin Y.-J. Umehara T. Inoue M. Saito K. Kigawa T. Jang M.K. Ozato K. Yokoyama S. Padmanabhan B. Guentert P.
TitleSolution structure of the extraterminal domain of the bromodomain-containing protein BRD4.
SourceProtein Sci. 17:2174-2179(2008).
PubMed ID18815416

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