PROSITE documentation PDOC51535Pestivirus NS3 protease (NS3pro) domain profile
The genome of pestiviruses [E1] is a single plus-strand RNA and contains a single large open reading frame (ORF), a 5' untranslated region (5'UTR) and a 3' untranslated region (3'UTR). The ORF encodes a polyprotein, which is processed by viral and cellular proteases into mature proteins (the structural proteins in the N-terminal portion of the polyprotein whilst the replicative (non-structural or NS) proteins constitute the remainder). The pestivirus NS3 protein is a multifunctional protein possessing serine protease, RNA helicase (see <PDOC51192>), and a nucleoside triphosphatase (NTPase) activities located in two functionally distinct domains. The N-terminal one-third of pestiviral NS3 primarily serves as a protease to process the viral polyprotein. The helicase and NTPase activities are localized to the C-terminal of NS3 protein. The NS3 protease (NS3pro) domain is of some 180 aa with a catalytic triad of His-Asp-Ser. Positive regulation (activation) is observed by its interaction with NS4A. The pestivirus NS3pro domain forms peptidase family S31 of clan PA [E2].
The profile we developed covers the entire pestivirus NS3pro domain.
Last update:May 2011 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Gorbalenya A.E. Donchenko A.P. Koonin E.V. Blinov V.M. |
| Title | N-terminal domains of putative helicases of flavi- and pestiviruses may be serine proteases. | |
| Source | Nucleic Acids Res. 17:3889-3897(1989). | |
| PubMed ID | 2543956 |
| 2 | Authors | Ryan M.D. Monaghan S. Flint M. |
| Title | Virus-encoded proteinases of the Flaviviridae. | |
| Source | J. Gen. Virol. 79:947-959(1998). | |
| PubMed ID | 9603310 |
| 3 | Authors | Wang P. Wang Y. Zhao Y. Zhu Z. Yu J. Wan L. Chen J. Xiao M. |
| Title | Classical swine fever virus NS3 enhances RNA-dependent RNA polymerase activity by binding to NS5B. | |
| Source | Virus Res. 148:17-23(2010). | |
| PubMed ID | 19951725 | |
| DOI | 10.1016/j.virusres.2009.11.015 |
| E1 | Title | https://viralzone.expasy.org/39?outline=all_by_species |
| E2 | Title | https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=s31 |
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