|PROSITE documentation PDOC51659|
The fucosylation of glycoconjugates in mammalian organisms is related to a wide variety of biological preocesses, including cell adhesion, blood antigens, and some severe diseases including cancer metastasis, congenital disorders of glycosylation, and various microbial and virus infections. Fucosylation via α1,2-, α1,3- α1,4-, and α1,6-linkages, and protein O-fucosylation are accomplished by the cation of specific individual fucosyltransferases .
FUT8, a eukaryotic α1,6-fucosyltransferase, catalyzes the transfer of a fucosyl residue from guanine nucleotide diphosphate (GDP)-β-L-fucose to the reducing terminal N-acetylglucosamine (GlcNAc) of asparagine-linked oligosaccharides (N-glycan). The catalytic domain of FUT8 is structurally similar to that of NodZ, a bacterial α1,6-fucosyltransferase. NodZ plays a role in the synthesis of the Nod factor, which is involved in the nodulation of legume roots for nitrogen fixing, and is known to catalyze the α1,6-fucosylation of lipo-chitooligosaccharides and variations thereof, including chitooligosaccharides. Both the eukaryotic and bacterial fucoslytransferase are classified into the GT23 family of Carbohydrate-Active enZYmes [E1] and share GDP-β-L-fucose as the donor substrate. Although the acceptor substrates are different, a "common" chitobiose unit is contained in the reducing terminals of both substrates .
The GT23 domain is comprised of two structures, a N-terminal open sheet α/ β structure and a C-terminal Rossmann fold which is frequently found in nucleotide binding proteins including glycosyltransferases (see <PDB:2DE0>) [1,3,4].
The profile we developed covers the entire GT23 domain.Last update:
October 2012 / First entry.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Ihara H. Ikeda Y. Toma S. Wang X. Suzuki T. Gu J. Miyoshi E. Tsukihara T. Honke K. Matsumoto A. Nakagawa A. Taniguchi N.|
|Title||Crystal structure of mammalian alpha1,6-fucosyltransferase, FUT8.|
|2||Authors||Ihara H. Hanashima S. Okada T. Ito R. Yamaguchi Y. Taniguchi N. Ikeda Y.|
|Title||Fucosylation of chitooligosaccharides by human alpha1,6-fucosyltransferase requires a nonreducing terminal chitotriose unit as a minimal structure.|
|3||Authors||Brzezinski K. Stepkowski T. Panjikar S. Bujacz G. Jaskolski M.|
|Title||High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor.|
|Source||Acta Biochim. Pol. 54:537-549(2007).|
|4||Authors||Brzezinski K. Dauter Z. Jaskolski M.|
|Title||Structures of NodZ alpha1,6-fucosyltransferase in complex with GDP and GDP-fucose.|
|Source||Acta Crystallogr. D 68:160-168(2012).|