Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC51694Peptidase family M66 domain profile
View entry in original PROSITE document format
View entry in raw text format (no links)
PURL: https://purl.expasy.org/prosite/documentation/PDOC51694
The peptidase family M66 [E1] domain is a zinc metalloprotease found in:
- Enterohemorrhagic Escherichia coli (EHEC) neutral zinc metalloprotease StcE (EC 3.4.24.-).
- Aeromonas hydrophila ToxR-regulated lipoprotein TagA.
- Vibrio cholerae ToxR-activated gene A lipoprotein TagA (EC 3.4.24.-).
The peptidase family M66 domain adopts a characteristic mixed α+β and a conserved Met-turn (see <PDB:3UJZ>), features that place it within the metzincin superfamily of metalloproteases including astacins, ADAMs (a disintegrin and metalloprotease) (see <PDOC50215>) and matrixins. Notably, a central substrate-binding cleft containing the active site separates the peptidase family M66 domain into two subdomains SD1 and SD2. SD1 consists of a twisted β sheet packed on both sides by three helices. A sharp turn introduced by a conserved Gly in the zinc-binding motif along the active site helix then leads to SD2, which, after a series of convoluted loops, terminates in an helix. The peptidase family M66 coordinates an essential zinc ion in the characteristic tetrahedral fashion and possesses the extended zinc-binding motif HExxHxxGxxH [1].
The profile we developed covers the entire peptidase family M66 domain.
Last update:October 2013 / First entry.
-------------------------------------------------------------------------------
PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Yu A.C.Y. Worrall L.J. Strynadka N.C.J. |
| Title | Structural insight into the bacterial mucinase StcE essential to adhesion and immune evasion during enterohemorrhagic E. coli infection. | |
| Source | Structure 20:707-717(2012). | |
| PubMed ID | 22483117 | |
| DOI | 10.1016/j.str.2012.02.015 |
| E1 | Title | https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=M66 |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.