PROSITE documentation PDOC51774
Networked (NET) actin-binding (NAB) domain profile


The Networked (NET) proteins are a superfamily of plant-specific actin-binding proteins which localize simultaneously to the actin cytoskeleton and specific membrane compartments and are suggested to couple these membranes to the actin cytoskeleton in plant cells. The minimal actin binding region, referred to as the NET actin-binding (NAB) domain, represents a new actin binding motif unique to plants with no apparent primary sequence homolgy to previously identified actin binding domains. In Arabidopsis, the NAB domain always starts with three conserved tryptophan residues, WWW, a motif whose worldwide web connection gives added significance to the NET family name. The C terminal half of the domain is very highly conserved, more so than the N terminal [1,2].

The predicted secondary structure of the domain includes three major α helices connected by a β turns with the WWW motif predicted to form a β sheet [1,2].

The profile we developed covers the entire NAB domain.

Last update:

September 2015 / First entry.


Technical section

PROSITE method (with tools and information) covered by this documentation:

NAB, PS51774; Networked (NET) actin-binding (NAB) domain profile  (MATRIX)


1AuthorsDeeks M.J. Calcutt J.R. Ingle E.K.S. Hawkins T.J. Chapman S. Richardson A.C. Mentlak D.A. Dixon M.R. Cartwright F. Smertenko A.P. Oparka K. Hussey P.J.
TitleA superfamily of actin-binding proteins at the actin-membrane nexus of higher plants.
SourceCurr. Biol. 22:1595-1600(2012).
PubMed ID22840520

2AuthorsHawkins T.J. Deeks M.J. Wang P. Hussey P.J.
TitleThe evolution of the actin binding NET superfamily.
SourceFront. Plant Sci. 5:254-254(2014).
PubMed ID24926301

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