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PROSITE documentation PDOC51889Rubella virus (RUBV) nonstructural (NS) protease domain profile
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PURL: https://purl.expasy.org/prosite/documentation/PDOC51889
Rubella virus (RUBV) is the etiological agent of a disease known as rubella or German measles. RUBV, a positive sense, single-strand RNA virus, is the sole member of the Rubivirus genus [E1] in the Togaviridae family [E2] of animal viruses. The RUBV genome contains two open reading frames (ORFs) encoding five proteins after postranslational proteolytic processing; two proteins (P150 and P90) involved in viral replication are translated from the nonstructural protein ORF (NSP-ORF) and the three structural proteins that form the virus particle are translated from the structural protein ORF (ST-ORF). A cysteine protease domain situated at the C-terminus of P150 and immediately upstream from the cleavage site within the NS-ORF precursor (P200) is responsible for self-cleavage of P200 into P150 and P90. This protease domain, termed the NS protease domain, is a papain-like protease with catalytic dyad of Cys and His. The NS protease domain contains an EF-hand Ca(2+) binding motif, which plays a structural role in stabilizing the protease at physiological temperatures, as well as a structural Zn(2+) binding site coordinated by four cysteines, which is necessary for protease activity. The RUBV NS protease domain forms peptidase family C27 [E3] of clan CA [1,2,3,4].
The profile we developed covers the entire RUBV NS protease domain.
Last update:April 2019 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Chen J.P. Strauss J.H. Strauss E.G. Frey T.K. |
| Title | Characterization of the rubella virus nonstructural protease domain and its cleavage site. | |
| Source | J. Virol. 70:4707-4713(1996). | |
| PubMed ID | 8676497 |
| 2 | Authors | ten Dam E. Flint M. Ryan M.D. |
| Title | Virus-encoded proteinases of the Togaviridae. | |
| Source | J. Gen. Virol. 80:1879-1888(1999). | |
| PubMed ID | 10466783 | |
| DOI | 10.1099/0022-1317-80-8-1879 |
| 3 | Authors | Liu X. Yang J. Ghazi A.M. Frey T.K. |
| Title | Characterization of the zinc binding activity of the rubella virus nonstructural protease. | |
| Source | J. Virol. 74:5949-5956(2000). | |
| PubMed ID | 10846076 |
| 4 | Authors | Zhou Y. Tzeng W.-P. Wong H.-C. Ye Y. Jiang J. Chen Y. Huang Y. Suppiah S. Frey T.K. Yang J.J. |
| Title | Calcium-dependent association of calmodulin with the rubella virus nonstructural protease domain. | |
| Source | J. Biol. Chem. 285:8855-8868(2010). | |
| PubMed ID | 20086014 | |
| DOI | 10.1074/jbc.M109.097063 |
| E1 | Title | https://viralzone.expasy.org/626?outline=all_by_species |
| E2 | Title | https://viralzone.expasy.org/3?outline=all_by_species |
| E3 | Title | https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=C27 |
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