PROSITE documentation PDOC51889
Rubella virus (RUBV) nonstructural (NS) protease domain profile


Rubella virus (RUBV) is the etiological agent of a disease known as rubella or German measles. RUBV, a positive sense, single-strand RNA virus, is the sole member of the Rubivirus genus [E1] in the Togaviridae family [E2] of animal viruses. The RUBV genome contains two open reading frames (ORFs) encoding five proteins after postranslational proteolytic processing; two proteins (P150 and P90) involved in viral replication are translated from the nonstructural protein ORF (NSP-ORF) and the three structural proteins that form the virus particle are translated from the structural protein ORF (ST-ORF). A cysteine protease domain situated at the C-terminus of P150 and immediately upstream from the cleavage site within the NS-ORF precursor (P200) is responsible for self-cleavage of P200 into P150 and P90. This protease domain, termed the NS protease domain, is a papain-like protease with catalytic dyad of Cys and His. The NS protease domain contains an EF-hand Ca(2+) binding motif, which plays a structural role in stabilizing the protease at physiological temperatures, as well as a structural Zn(2+) binding site coordinated by four cysteines, which is necessary for protease activity. The RUBV NS protease domain forms peptidase family C27 [E3] of clan CA [1,2,3,4].

The profile we developed covers the entire RUBV NS protease domain.

Last update:

April 2019 / First entry.


Technical section

PROSITE method (with tools and information) covered by this documentation:

RUBV_NS_PRO, PS51889; Rubella virus (RUBV) nonstructural (NS) protease domain profile  (MATRIX)


1AuthorsChen J.P. Strauss J.H. Strauss E.G. Frey T.K.
TitleCharacterization of the rubella virus nonstructural protease domain and its cleavage site.
SourceJ. Virol. 70:4707-4713(1996).
PubMed ID8676497

2Authorsten Dam E. Flint M. Ryan M.D.
TitleVirus-encoded proteinases of the Togaviridae.
SourceJ. Gen. Virol. 80:1879-1888(1999).
PubMed ID10466783

3AuthorsLiu X. Yang J. Ghazi A.M. Frey T.K.
TitleCharacterization of the zinc binding activity of the rubella virus nonstructural protease.
SourceJ. Virol. 74:5949-5956(2000).
PubMed ID10846076

4AuthorsZhou Y. Tzeng W.-P. Wong H.-C. Ye Y. Jiang J. Chen Y. Huang Y. Suppiah S. Frey T.K. Yang J.J.
TitleCalcium-dependent association of calmodulin with the rubella virus nonstructural protease domain.
SourceJ. Biol. Chem. 285:8855-8868(2010).
PubMed ID20086014




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