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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

PROSITE entry PS00433


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PURL: https://purl.expasy.org/prosite/signature/PS00433

Direct ScanProsite submission
General information about the entry

Entry name [info] PHOSPHOFRUCTOKINASE
Accession [info] PS00433
Entry type [info] PATTERN
Date [info] 01-NOV-1990 CREATED;
01-APR-2006 DATA UPDATE;
29-MAY-2024 INFO UPDATE.
PROSITE Doc. [info] PDOC00336

Name and characterization of the entry

Description [info] Phosphofructokinase signature.
Pattern [info] 
[RK]-x(4)-[GAS]-H-x-[QL]-[QR]-[GS]-[GF]-x(5)-[DE]-[RL].

Numerical results [info]

Numerical results for UniProtKB/Swiss-Prot release 2025_04 which contains 573'661 sequence entries.


Total number of hits 340 in 303 different sequences
Number of true positive hits 340 in 303 different sequences
Number of 'unknown' hits 0
Number of false positive hits 0
Number of false negative sequences 17
Number of 'partial' sequences 2
Precision (true positives / (true positives + false positives)) 100.00 %
Recall (true positives / (true positives + false negatives)) 95.24 %

Comments [info]

Taxonomic range [info] Archaea, Eukaryotes, Prokaryotes (Bacteria)
Maximum number of repetitions [info] 2
Site [info] substrate_binding at position 1
Site [info] substrate_binding at position 4
Site [info] substrate_binding at position 7
Version [info] 2

Cross-references [info]

UniProtKB/Swiss-Prot
True positive sequences		 303 sequences 

PFKA1_ASPOR (Q9HGZ1), PFKA1_BACTN (Q8A8R5), PFKA1_CAEEL (Q9TZL8), 

PFKA1_CANAX (O94201), PFKA1_KLULA (Q03215), PFKA1_KOMPG (Q92448), 
PFKA1_LACLL (P0DOB6), PFKA1_NOSS1 (Q8YKG3), PFKA1_PICPA (Q8NJU8), 
PFKA1_SYNY3 (P72830), PFKA1_THET8 (P21777), PFKA1_YEAST (P16861), 
PFKA2_ASPOR (Q9HGZ0), PFKA2_BACTN (Q8A624), PFKA2_CAEEL (Q27483), 
PFKA2_CANAX (O94200), PFKA2_CLOPE (Q8XL57), PFKA2_KLULA (Q03216), 
PFKA2_KOMPG (C4QXA5), PFKA2_LACLL (P0DOB7), PFKA2_NOSS1 (Q8YVR1), 
PFKA2_PICPA (Q8TGA0), PFKA2_STRCO (Q9L1L8), PFKA2_SYNY3 (Q55988), 
PFKA2_YEAST (P16862), PFKA3_STRCO (Q9FC99), PFKAL_BOVIN (A1A4J1), 
PFKAL_HUMAN (P17858), PFKAL_MOUSE (P12382), PFKAL_PONAB (Q5R7V5), 
PFKAL_RAT   (P30835), PFKAM_BOVIN (Q0IIG5), PFKAM_CANLF (P52784), 
PFKAM_HORSE (Q867C9), PFKAM_HUMAN (P08237), PFKAM_MACFA (Q60HD9), 
PFKAM_MOUSE (P47857), PFKAM_PIG   (Q2HYU2), PFKAM_PONAB (Q5RAG9), 
PFKAM_RABIT (P00511), PFKAM_RAT   (P47858), PFKAP_HUMAN (Q01813), 
PFKAP_MOUSE (Q9WUA3), PFKAP_PONAB (Q5R636), PFKAP_RABIT (P47859), 
PFKAP_RAT   (P47860), PFKA_ACTP2  (A3N1C8), PFKA_ACTP7  (B3GY11), 
PFKA_ACTPJ  (B0BQ64), PFKA_ACTSZ  (A6VM63), PFKA_ALIF1  (Q5E2B1), 
PFKA_ALIFM  (B5FBT1), PFKA_ALISL  (B6EMS4), PFKA_ALKMQ  (A6TVD3), 
PFKA_ALKOO  (A8MLY0), PFKA_ANOFW  (B7GGT1), PFKA_AQUAE  (O67605), 
PFKA_ASPFU  (Q4W9B8), PFKA_ASPNG  (P78985), PFKA_BACAA  (C3PAI8), 
PFKA_BACAC  (C3L8X8), PFKA_BACAH  (A0RJJ6), PFKA_BACAN  (Q81KZ0), 
PFKA_BACC0  (B7JRX2), PFKA_BACC1  (Q72ZD8), PFKA_BACC2  (B7IJZ8), 
PFKA_BACC3  (C1EUU3), PFKA_BACC4  (B7HFB3), PFKA_BACC7  (B7HRN9), 
PFKA_BACCE  (Q4MVY3), PFKA_BACCN  (A7GTP4), PFKA_BACCQ  (B9J099), 
PFKA_BACCR  (Q817F3), PFKA_BACCZ  (Q633J8), PFKA_BACHK  (Q6HCT3), 
PFKA_BACLD  (Q65G82), PFKA_BACMK  (A9VJR1), PFKA_BACP2  (A8FG55), 
PFKA_BACSU  (O34529), PFKA_BACVZ  (A7Z7K6), PFKA_BAUCH  (Q1LTT2), 
PFKA_BLOFL  (Q7VRK9), PFKA_BREBN  (C0Z7W7), PFKA_BUCA5  (B8D996), 
PFKA_BUCAI  (P57391), PFKA_BUCAP  (Q8K9N0), PFKA_BUCAT  (B8D7J8), 
PFKA_BUCBP  (P59563), PFKA_BUCCC  (Q057P0), PFKA_CALS4  (Q8R914), 
PFKA_CHLAA  (A9WCU2), PFKA_CHLAD  (B8GAA4), PFKA_CHLSY  (B9LHK4), 
PFKA_CITK8  (A8AL13), PFKA_CLOAB  (O08308), PFKA_CLOB1  (A7FYW7), 
PFKA_CLOB6  (C3KV06), PFKA_CLOBA  (B2UZX8), PFKA_CLOBB  (B2TQR6), 
PFKA_CLOBH  (A5I798), PFKA_CLOBJ  (C1FM55), PFKA_CLOBK  (B1IFV9), 
PFKA_CLOBL  (A7GIW9), PFKA_CLOBM  (B1L266), PFKA_CLOD6  (Q180P4), 
PFKA_CLONN  (A0PYC8), PFKA_CLOTE  (Q890Z2), PFKA_COREF  (Q8RQN5), 
PFKA_CORGL  (Q8NR14), PFKA_COXBU  (Q83EH9), PFKA_CROS8  (A7MQ83), 
PFKA_CYTH3  (Q11RE2), PFKA_DEIRA  (Q9RWN1), PFKA_DICDI  (P90521), 
PFKA_DROME  (P52034), PFKA_ECO24  (A7ZUC9), PFKA_ECO27  (B7UNN6), 
PFKA_ECO45  (B7MI48), PFKA_ECO55  (B7LA13), PFKA_ECO57  (P0A797), 
PFKA_ECO5E  (B5YZ53), PFKA_ECO7I  (B7NU91), PFKA_ECO81  (B7N2Q7), 
PFKA_ECO8A  (B7M6W7), PFKA_ECOBW  (C5A083), PFKA_ECODH  (B1XB82), 
PFKA_ECOHS  (A8A720), PFKA_ECOL5  (Q0TAE8), PFKA_ECOL6  (Q8FBD0), 
PFKA_ECOLC  (B1IVG3), PFKA_ECOLI  (P0A796), PFKA_ECOLU  (B7NFL3), 
PFKA_ECOSE  (B6I4Q8), PFKA_ECOSM  (B1LNL9), PFKA_EDWI9  (C5BC26), 
PFKA_ENT38  (A4WG81), PFKA_ENTCL  (Q9Z6C3), PFKA_ERWT9  (B2VF62), 
PFKA_ESCF3  (B7LVC9), PFKA_FINM2  (B0S230), PFKA_FUSNN  (Q8RG98), 
PFKA_GEOKA  (Q5KWB1), PFKA_GEOSE  (P00512), PFKA_GEOSW  (C5D663), 
PFKA_GLAP5  (B8F8G9), PFKA_HAECO  (Q27665), PFKA_HAEDU  (Q8GNC1), 
PFKA_HAEI8  (Q4QLS8), PFKA_HAEIE  (A5UD89), PFKA_HAEIN  (P43863), 
PFKA_HALH5  (Q9K843), PFKA_HALOH  (B8D1K5), PFKA_HAMD5  (C4K7E1), 
PFKA_HISS1  (Q0I267), PFKA_HISS2  (B0UW05), PFKA_KLEP3  (B5XZ40), 
PFKA_KLEP7  (A6TGB8), PFKA_LACAC  (Q5FKG6), PFKA_LACDE  (P80019), 
PFKA_LACGA  (Q043V2), PFKA_LACH4  (A8YV22), PFKA_LACJO  (Q74JM8), 
PFKA_LACLA  (P0DOB5), PFKA_LACLM  (A2RKA2), PFKA_LACLS  (Q02YK5), 
PFKA_LACPL  (Q88VY1), PFKA_LIGS1  (Q1WTT3), PFKA_LISIN  (Q92BE4), 
PFKA_LISMC  (C1KVL8), PFKA_LISMF  (Q71Z96), PFKA_LISMH  (B8DHH7), 
PFKA_LISMO  (Q8Y6W0), PFKA_LISW6  (A0AJ20), PFKA_LYSSH  (Q93LR4), 
PFKA_MACCJ  (B9E7A4), PFKA_MANSM  (Q65VM6), PFKA_MESH2  (Q601D3), 
PFKA_MESH7  (Q4A8Q3), PFKA_MESHJ  (Q4AAM2), PFKA_MYCBO  (P65691), 
PFKA_MYCCT  (Q2SSQ6), PFKA_MYCLE  (O33106), PFKA_MYCM1  (Q6KHX0), 
PFKA_MYCPU  (Q98PW8), PFKA_MYCTO  (P9WID6), PFKA_MYCTU  (P9WID7), 
PFKA_NATTJ  (B2A6Y0), PFKA_OCEIH  (Q8EPD6), PFKA_PAEMC  (Q59214), 
PFKA_PASMU  (Q9CPH2), PFKA_PECAS  (Q6CZ46), PFKA_PECCP  (C6DHK2), 
PFKA_PEDPA  (Q03F74), PFKA_PHOPR  (Q6LVK2), PFKA_SALA4  (B5F0P6), 
PFKA_SALAR  (A9MI50), PFKA_SALCH  (Q57HF3), PFKA_SALDC  (B5FPR2), 
PFKA_SALEP  (B5QWZ5), PFKA_SALG2  (B5RFA9), PFKA_SALHS  (B4TCK0), 
PFKA_SALNS  (B4T049), PFKA_SALPA  (Q5PIR6), PFKA_SALPB  (A9MZE7), 
PFKA_SALPC  (C0Q414), PFKA_SALPK  (B5BJI2), PFKA_SALSV  (B4TPS6), 
PFKA_SALTI  (P65693), PFKA_SALTY  (P65692), PFKA_SCHMA  (Q27778), 
PFKA_SCHPO  (O42938), PFKA_SERP5  (A8GLB0), PFKA_SHIB3  (B2TVQ8), 
PFKA_SHIBS  (Q31U74), PFKA_SHIDS  (Q32A80), PFKA_SHIFL  (P0A798), 
PFKA_SHISS  (Q3YV62), PFKA_SHOC1  (Q5WEF6), PFKA_SODGM  (Q2NQX2), 
PFKA_SPICI  (P20275), PFKA_STAA2  (A6U2G5), PFKA_STAA8  (Q2FXM8), 
PFKA_STAA9  (A5ITM2), PFKA_STAAB  (Q2YTE2), PFKA_STAAC  (Q5HF75), 
PFKA_STAAE  (A6QHN3), PFKA_STAAM  (P65694), PFKA_STAAN  (P99165), 
PFKA_STAAR  (Q6GG08), PFKA_STAAS  (Q6G8M8), PFKA_STAAT  (A8Z2L4), 
PFKA_STAAW  (P65695), PFKA_STACT  (B9DN94), PFKA_STAEQ  (Q5HNK6), 
PFKA_STAES  (Q8CS68), PFKA_STRA1  (Q3K1E6), PFKA_STRA3  (P65696), 
PFKA_STRA5  (P65697), PFKA_STRE4  (C0M8V6), PFKA_STREM  (B4U2M2), 
PFKA_STRGC  (A8AXW1), PFKA_STRMU  (Q8DTX6), PFKA_STRP1  (Q99ZD0), 
PFKA_STRP2  (Q04L24), PFKA_STRP3  (P0DD02), PFKA_STRP4  (B5E3Z8), 
PFKA_STRP6  (Q5XBV2), PFKA_STRP7  (C1C6M8), PFKA_STRP8  (Q8P0S6), 
PFKA_STRPB  (Q1JBF7), PFKA_STRPC  (Q1JLD9), PFKA_STRPD  (Q1JGH6), 
PFKA_STRPF  (Q1J694), PFKA_STRPG  (A2RE65), PFKA_STRPI  (B1IB66), 
PFKA_STRPJ  (B8ZNU0), PFKA_STRPM  (Q48T86), PFKA_STRPN  (Q97RC6), 
PFKA_STRPQ  (P0DD03), PFKA_STRPS  (B2IP53), PFKA_STRPZ  (B5XLV6), 
PFKA_STRR6  (Q8DQ85), PFKA_STRS7  (C0MGJ9), PFKA_STRSV  (A3CM69), 
PFKA_STRSY  (A4VTS0), PFKA_STRU0  (B9DSA4), PFKA_STRZJ  (C1CDP4), 
PFKA_STRZP  (C1CJY4), PFKA_STRZT  (C1CRZ7), PFKA_SULDN  (Q30T53), 
PFKA_SULNB  (A6QAB4), PFKA_TERTT  (C5BNR1), PFKA_THEMA  (Q9WY52), 
PFKA_THENN  (B9K6R9), PFKA_THEP1  (A5IKL2), PFKA_THEP3  (B0K7U7), 
PFKA_THEPX  (B0K6L2), PFKA_THESQ  (B1L9U3), PFKA_THET2  (Q72H98), 
PFKA_THEVB  (Q8DJB1), PFKA_TOLAT  (C4LEQ5), PFKA_TRIV2  (Q3M8N2), 
PFKA_VIBC1  (A7MX59), PFKA_VIBCH  (Q9KNP2), PFKA_VIBPA  (Q87KX0), 
PFKA_VIBVU  (Q8DCY1), PFKA_VIBVY  (Q7MGW6), PFKA_WOLSU  (Q7M9C2), 
PFKA_YARLI  (P59680), PFKA_YERE8  (A1JHZ3), PFKA_YERP3  (A7FCW1), 
PFKA_YERPA  (Q1C297), PFKA_YERPB  (B2JZA3), PFKA_YERPE  (Q8ZJL6), 
PFKA_YERPG  (A9R6A3), PFKA_YERPN  (Q1CD33), PFKA_YERPP  (A4TSA7), 
PFKA_YERPS  (Q66GA8), PFKA_YERPY  (B1JQU4), PFP_AMYME   (Q59126), 
PFP_AMYMS   (Q9AGC0), PFP_DICTH   (Q9KH71), PFP_STRCO   (O08333)
» more

UniProtKB/Swiss-Prot
False negative sequences		 17 sequences 

PFKA1_CLOPE (Q8XNH2), PFKA3_BACTN (Q8A2E9), PFKA_ENTFA  (Q836R3), 

PFKA_LACCB  (B3WE64), PFKA_LACP3  (Q039I0), PFKA_MALP2  (Q8EVH2), 
PFKA_MYCGE  (P47457), PFKA_MYCPN  (P75476), PFKA_PERMH  (C0QQX4), 
PFKA_PROFC  (D7GCU5), PFKA_SPITD  (E0RTD9), PFKA_STRTD  (Q03KB7), 
PFKA_STRTR  (Q9L9E3), PFKA_SULSY  (B2V7W7), PFKA_UREPA  (Q9PQV8), 
PFP_THETK   (G4RK16), SQIK_ALKUR  (A0A2P6MHT4)
» more

UniProtKB/Swiss-Prot
'Partial' sequences		 2 sequences 

PFKA2_THET8 (P21778), PFKA_CALFI  (Q27543)


				
	

	

		PDB
[Detailed view]		
		
			35 PDB
			


			pdb_00001mto; pdb_00001pfk; pdb_00001zxx; pdb_00002pfk; pdb_00003o8l; pdb_00003o8n; pdb_00003o8o; pdb_00003opy; pdb_00003pfk; pdb_00003u39; pdb_00004a3s; pdb_00004i36; 
			
				pdb_00004i4i; pdb_00004i7e; pdb_00004omt; pdb_00004pfk; pdb_00004rh3; pdb_00004u1r; pdb_00004wl0; pdb_00004xyj; pdb_00004xyk; pdb_00004xz2; pdb_00005xoe; pdb_00005xz6; 
				pdb_00005xz7; pdb_00005xz8; pdb_00005xz9; pdb_00005xza; pdb_00006pfk; pdb_00007lw1; pdb_00007tff; pdb_00008w2g; pdb_00008w2h; pdb_00008w2i; pdb_00008w2j
			
			

			» more