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PROSITE documentation PDOC00130 [for PROSITE entry PS00143] |
A number of proteases dependent on divalent cations for their activity have been shown [1,2] to belong to one family, on the basis of sequence similarity. These enzymes are listed below.
It should be noted that in addition to the above enzymes, this family also includes the core proteins I and II of the mitochondrial bc1 complex (also called cytochrome c reductase or complex III), but the situation as to the activity or lack of activity of these subunits is quite complex:
These proteins do not share many regions of sequence similarity; the most noticeable is in the N-terminal section. This region includes a conserved histidine followed, two residues later by a glutamate and another histidine. In pitrilysin, it has been shown [4] that this H-x-x-E-H motif is involved in enzyme activity; the two histidines bind zinc and the glutamate is necessary for catalytic activity. Non active members of this family have lost from one to three of these active site residues. We developed a signature pattern that detect active members of this family as well as some inactive members.
Note:These proteins belong to family M16 in the classification of peptidases [5,E1].
Last update:May 2004 / Text revised.
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PROSITE method (with tools and information) covered by this documentation:
1 | Authors | Rawlings N.D. Barrett A.J. |
Title | Homologues of insulinase, a new superfamily of metalloendopeptidases. | |
Source | Biochem. J. 275:389-391(1991). | |
PubMed ID | 2025223 |
2 | Authors | Braun H.-P. Schmitz U.K. |
Title | Are the 'core' proteins of the mitochondrial bc1 complex evolutionary relics of a processing protease? | |
Source | Trends Biochem. Sci. 20:171-175(1995). | |
PubMed ID | 7610476 |
3 | Authors | Becker A.B. Roth R.A. |
Title | An unusual active site identified in a family of zinc metalloendopeptidases. | |
Source | Proc. Natl. Acad. Sci. U.S.A. 89:3835-3839(1992). | |
PubMed ID | 1570301 |
4 | Authors | Fujita A. Oka C. Arikawa Y. Katagai T. Tonouchi A. Kuhara S. Misumi Y. |
Title | A yeast gene necessary for bud-site selection encodes a protein similar to insulin-degrading enzymes. | |
Source | Nature 372:567-570(1994). | |
PubMed ID | 7990931 | |
DOI | 10.1038/372567a0 |
5 | Authors | Rawlings N.D. Barrett A.J. |
Title | Evolutionary families of metallopeptidases. | |
Source | Methods Enzymol. 248:183-228(1995). | |
PubMed ID | 7674922 |
E1 | Title | https://www.uniprot.org/docs/peptidas |