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PROSITE documentation PDOC00479 [for PROSITE entry PS00554]

TEA domain signature and profile


The TEA domain is a DNA-binding region of ~80 amino acids that has been named after the two proteins that originally defined the domain: TEF-1 and AbaA. It is also referred to as the ATTS domain because of the 4 proteins first found to harbor this motif (AbaA and TEC1 in yeast, TEF1/TEAD1 in vertebrates and Scalloped in fly). The TEA domain is located toward the amino terminus of eukaryotic transcription factors of the TEA/ATTS family. The consensus DNA sequence bound by an isolated TEA domain is N-[ATG]-G-[ATC]-A-T-N-T; where, N=A, T, C. or G [1,2,3,4].

The TEA domain is composed of a three-helix bundle with a homeodomain fold (see <PDB:2HZD>). It has a folded globular structure made of three α-helices, H1, H2, and H3. H1 and H2 are nearly anti-parallel and pack on either side of the H3, which is the DNA-recognition helix of the TEA domain. Two conserved serines are found on the DNA-binding surface. Phosphorylation of one or both of these could interfere with DNA-binding activity, by introducing electrostatic repulsion and/or steric hindrance, and help regulate TEA/ATTS transcription factor activity [3,4].

Some of the proteins that contain a TEA domain are listed below:

  • Mammalian enhancer factor TEF-1. TEF-1 can bind to two distinct sequences in the SV40 enhancer and is a transcriptional activator.
  • Mammalian TEF-3, TEF-4 and TEF-5 [5], putative transcriptional activators highly similar to TEF-1.
  • Drosophila scalloped protein (gene sd), a probable transcription factor that functions in the regulation of cell-specific gene expression during Drosophila development, particularly in the differentiation of the nervous system [6].
  • Emericella nidulans regulatory protein abaA. AbaA is involved in the regulation of conidiation (asexual spore); its expression leads to the cessation of vegetative growth.
  • Yeast trans-acting factor TEC1. TEC1 is involved in the activation of the Ty1 retrotransposon.
  • Caenorhabditis elegans hypothetical protein F28B12.2.

We developed both a pattern and a profile. The pattern covers helices H2 and H3, whereas the profile covers the whole domain.

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html --------------------------------------------------------------------------------.

Last update:

January 2017 / Profile and text revised.


Technical section

PROSITE methods (with tools and information) covered by this documentation:

TEA_1, PS00554; TEA domain signature  (PATTERN)

TEA_2, PS51088; TEA domain profile  (MATRIX)


1AuthorsBuerglin T.R.
TitleThe TEA domain: a novel, highly conserved DNA-binding motif.
SourceCell 66:11-12(1991).
PubMed ID2070413

2AuthorsHwang J.J. Chambon P. Davidson I.
TitleCharacterization of the transcription activation function and the DNA binding domain of transcriptional enhancer factor-1.
SourceEMBO J. 12:2337-2348(1993).
PubMed ID8389695

3AuthorsAnbanandam A. Albarado D.C. Nguyen C.T. Halder G. Gao X. Veeraraghavan S.
TitleInsights into transcription enhancer factor 1 (TEF-1) activity from the solution structure of the TEA domain.
SourceProc. Natl. Acad. Sci. U.S.A. 103:17225-17230(2006).
PubMed ID17085591

4AuthorsLandin-Malt A. Benhaddou A. Zider A. Flagiello D.
TitleAn evolutionary, structural and functional overview of the mammalian TEAD1 and TEAD2 transcription factors.
SourceGene 591:292-303(2016).
PubMed ID27421669

5AuthorsJacquemin P. Hwang J.-J. Martial J.A. Dolle P. Davidson I.
TitleA novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain.
SourceJ. Biol. Chem. 271:21775-21785(1996).
PubMed ID8702974

6AuthorsCampbell S. Inamdar M. Rodrigues V. Raghavan V. Palazzolo M. Chovnick A.
TitleThe scalloped gene encodes a novel, evolutionarily conserved transcription factor required for sensory organ differentiation in Drosophila.
SourceGenes Dev. 6:367-379(1992).
PubMed ID1547938

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.


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