|PROSITE documentation PDOC00641 [for PROSITE entry PS00813]|
Eukaryotic translation initiation factor 4E (eIF-4E)  is a protein that binds to the cap structure of eukaryotic cellular mRNAs. eIF-4E recognizes and binds the 7-methylguanosine-containing (m7Gppp) cap during an early step in the initiation of protein synthesis and facilitates ribosome binding to a mRNA by inducing the unwinding of its secondary structures.
eIF-4E is a conserved protein of about 25 Kd. Site directed mutagenesis experiments have shown  that a tryptophan in the central part of the sequence of human eIF-4E seems to be implicated in cap-binding. The signature pattern for eIF-4E includes this tryptophan.Last update:
December 2004 / Pattern and text revised.
PROSITE method (with tools and information) covered by this documentation:
|Title||Cap recap: the involvement of eIF-4F in regulating gene expression.|
|2||Authors||Ueda H. Iyo H. Doi M. Inoue M. Ishida T. Morioka H. Tanaka T. Nishikawa S. Uesugi S.|
|Title||Combination of Trp and Glu residues for recognition of mRNA cap structure. Analysis of m7G base recognition site of human cap binding protein (IF-4E) by site-directed mutagenesis.|
|Source||FEBS Lett. 280:207-210(1991).|