PROSITE documentation PDOC00641
Eukaryotic initiation factor 4E signature


Eukaryotic translation initiation factor 4E (eIF-4E) [1] is a protein that binds to the cap structure of eukaryotic cellular mRNAs. eIF-4E recognizes and binds the 7-methylguanosine-containing (m7Gppp) cap during an early step in the initiation of protein synthesis and facilitates ribosome binding to a mRNA by inducing the unwinding of its secondary structures.

eIF-4E is a conserved protein of about 25 Kd. Site directed mutagenesis experiments have shown [2] that a tryptophan in the central part of the sequence of human eIF-4E seems to be implicated in cap-binding. The signature pattern for eIF-4E includes this tryptophan.

Last update:

December 2004 / Pattern and text revised.


Technical section

PROSITE method (with tools and information) covered by this documentation:

IF4E, PS00813; Eukaryotic initiation factor 4E signature  (PATTERN)


1AuthorsThach R.E.
TitleCap recap: the involvement of eIF-4F in regulating gene expression.
SourceCell 68:177-180(1992).
PubMed ID1733496

2AuthorsUeda H. Iyo H. Doi M. Inoue M. Ishida T. Morioka H. Tanaka T. Nishikawa S. Uesugi S.
TitleCombination of Trp and Glu residues for recognition of mRNA cap structure. Analysis of m7G base recognition site of human cap binding protein (IF-4E) by site-directed mutagenesis.
SourceFEBS Lett. 280:207-210(1991).
PubMed ID1672854

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