PROSITE documentation PDOC00641Eukaryotic initiation factor 4E signature
Eukaryotic translation initiation factor 4E (eIF-4E) [1] is a protein that binds to the cap structure of eukaryotic cellular mRNAs. eIF-4E recognizes and binds the 7-methylguanosine-containing (m7Gppp) cap during an early step in the initiation of protein synthesis and facilitates ribosome binding to a mRNA by inducing the unwinding of its secondary structures.
eIF-4E is a conserved protein of about 25 Kd. Site directed mutagenesis experiments have shown [2] that a tryptophan in the central part of the sequence of human eIF-4E seems to be implicated in cap-binding. The signature pattern for eIF-4E includes this tryptophan.
Last update:December 2004 / Pattern and text revised.
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| 1 | Authors | Thach R.E. |
| Title | Cap recap: the involvement of eIF-4F in regulating gene expression. | |
| Source | Cell 68:177-180(1992). | |
| PubMed ID | 1733496 |
| 2 | Authors | Ueda H. Iyo H. Doi M. Inoue M. Ishida T. Morioka H. Tanaka T. Nishikawa S. Uesugi S. |
| Title | Combination of Trp and Glu residues for recognition of mRNA cap structure. Analysis of m7G base recognition site of human cap binding protein (IF-4E) by site-directed mutagenesis. | |
| Source | FEBS Lett. 280:207-210(1991). | |
| PubMed ID | 1672854 |
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