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PROSITE documentation PDOC00708 [for PROSITE entry PS00911]

Dihydroorotate dehydrogenase signatures


Dihydroorotate dehydrogenase (EC (DHOdehase) catalyzes the fourth step in the de novo biosynthesis of pyrimidine, the conversion of dihydroorotate into orotate. DHOdehase is a ubiquitous FAD flavoprotein.

In bacteria (gene pyrD), DHOdease is located on the inner side of the cytosolic membrane. In some yeasts, such as in Saccharomyces cerevisiae (gene URA1), it is a cytosolic protein while in other eukaryotes it is found in the mitochondria [1].

The sequence of DHOdease is rather well conserved and we have developed two signature patterns specific to this enzyme. The first corresponds to a region in the N-terminal section of the enzyme while the second is located in the C-terminal section and seems to be part of the FAD-binding domain.

Last update:

April 2006 / Pattern revised.

Technical section

PROSITE methods (with tools and information) covered by this documentation:

DHODEHASE_1, PS00911; Dihydroorotate dehydrogenase signature 1  (PATTERN)

DHODEHASE_2, PS00912; Dihydroorotate dehydrogenase signature 2  (PATTERN)


1AuthorsNagy M. Lacroute F. Thomas D.
TitleDivergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeasts.
SourceProc. Natl. Acad. Sci. U.S.A. 89:8966-8970(1992).
PubMed ID1409592

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