|PROSITE documentation PDOC00708 [for PROSITE entry PS00911]|
Dihydroorotate dehydrogenase (EC 18.104.22.168) (DHOdehase) catalyzes the fourth step in the de novo biosynthesis of pyrimidine, the conversion of dihydroorotate into orotate. DHOdehase is a ubiquitous FAD flavoprotein.
In bacteria (gene pyrD), DHOdease is located on the inner side of the cytosolic membrane. In some yeasts, such as in Saccharomyces cerevisiae (gene URA1), it is a cytosolic protein while in other eukaryotes it is found in the mitochondria .
The sequence of DHOdease is rather well conserved and we have developed two signature patterns specific to this enzyme. The first corresponds to a region in the N-terminal section of the enzyme while the second is located in the C-terminal section and seems to be part of the FAD-binding domain.Last update:
April 2006 / Pattern revised.
PROSITE methods (with tools and information) covered by this documentation:
|1||Authors||Nagy M. Lacroute F. Thomas D.|
|Title||Divergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeasts.|
|Source||Proc. Natl. Acad. Sci. U.S.A. 89:8966-8970(1992).|