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	PROSITE documentation PDOC00772 [for PROSITE entry PS01009]

CRISP family signature

A variety of extracellular proteins from eukaryotes have been found to be evolutionary related. They have been termed cysteine-rich secretory proteins (CRISP). Some relevant members of this family are:

• Rodent sperm-coating glycoprotein (SCP), also known as acidic epididymal glycoprotein (AEG) . This protein is thought to be involved in sperm maturation [1]. It is a protein of about 220 residues and probably contains eight disulfide bonds.
• Mammalian testis-specific protein Tpx-1 [2]. Tpx-1 is highly related to SCP's.
• Mammalian glioma pathogenesis-related protein (GliPR).
• Lizard helothermine, a toxin that blocks ryanodine receptors.
• Venom allergen 5 (Ag5) from vespid wasps and venom allergen 3 (Ag3) from fire ants. These proteins are potent allergens and are the main cause of allergic reactions to stings from insects of the hymenoptera family [3]. Ag5/3 are proteins of about 200 residues and contain four disulfide bonds.
• Plant pathogenesis proteins of the PR-1 family [4]. These proteins are synthesized during pathogen infection or other stress-related responses. They are proteins of about 130 to 140 residues and probably contain three disulfide bonds.
• Proteins Sc7 and Sc14 from the basidomycete fungus Schizophyllum commune. These extracellular proteins are loosely associated with fruit body hyphal walls [5]. Sc7/14 are proteins of about 180 residues and probably contain two disulfide bonds.
• Ancylostoma secreted protein from dog hookworm.
• Yeast hypothetical proteins YJL078c, YJL079c and YKR013w.

The exact function of these proteins is not yet known. As signature patterns, we selected two conserved regions located in their C-terminal half. The second signature contains a cysteine which is known to be involved in a disulfide bond in Ag5.

Last update:

April 2006 / Pattern revised.

PROSITE methods (with tools and information) covered by this documentation:

CRISP_1, PS01009; CRISP family signature 1  (PATTERN)

• Consensus pattern:
  [GDER]-[HR]-[FYWH]-[TVS]-[QA]-[LIVM]-[LIVMA]-W-x(2)-[STN]
• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 173
  • detected by PS01009: 123 (true positives)
  • undetected by PS01009: 50 (19 false negatives and 31 'partials')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS01009:
  1 false positive.
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01009
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01009
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS01009
• View ligand binding statistics of PS01009
• Matching PDB structures: 1CFE 1QNX 1RC9 1SMB ... [ALL]

CRISP_2, PS01010; CRISP family signature 2  (PATTERN)

• Consensus pattern:
  [LIVMFYH]-[LIVMFY]-x-C-[NQRHS]-Y-x-[PARH]-x-[GL]-N-[LIVMFYWDN]
  C is involved in a disulfide bond
• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 174
  • detected by PS01010: 127 (true positives)
  • undetected by PS01010: 47 (17 false negatives and 30 'partials')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS01010:
  NONE.
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01010
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01010
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS01010
• View ligand binding statistics of PS01010
• Matching PDB structures: 1CFE 1QNX 1RC9 1WVR ... [ALL]

1	Authors	Mizuki N., Kasahara M.
	Title	Mouse submandibular glands express an androgen-regulated transcript encoding an acidic epididymal glycoprotein-like molecule.
	Source	Mol. Cell. Endocrinol. 89:25-32(1992).
	PubMed ID	1301383

2	Authors	Kasahara M., Gutknecht J., Brew K., Spurr N., Goodfellow P.N.
	Title	Cloning and mapping of a testis-specific gene with sequence similarity to a sperm-coating glycoprotein gene.
	Source	Genomics 5:527-534(1989).
	PubMed ID	2613236

3	Authors	Lu G., Villalba M., Coscia M.R., Hoffman D.R., King T.P.
	Title	Sequence analysis and antigenic cross-reactivity of a venom allergen, antigen 5, from hornets, wasps, and yellow jackets.
	Source	J. Immunol. 150:2823-2830(1993).
	PubMed ID	8454859

4	Authors	Dixon D.C., Cutt J.R., Klessig D.F.
	Title	Differential targeting of the tobacco PR-1 pathogenesis-related proteins to the extracellular space and vacuoles of crystal idioblasts.
	Source	EMBO J. 10:1317-1324(1991).
	PubMed ID	2026137

5	Authors	Schuren F.H.J., Asgeirsdottir S.A., Kothe E.M., Scheer J.M.J., Wessels J.G.
	Title	The Sc7/Sc14 gene family of Schizophyllum commune codes for extracellular proteins specifically expressed during fruit-body formation.
	Source	J. Gen. Microbiol. 139:2083-2090(1993).
	PubMed ID	8245835

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