|PROSITE documentation PDOC00821 [for PROSITE entry PS01070]|
A family of bacterial and eukaryotic endonucleases (EC 3.1.30.-) share the following characteristics: they act on both DNA and RNA, cleave double-stranded and single-standed nucleic acids and require a divalent ion such as magnesium for their activity. The enzymes that belongs to this family are:
An histidine has been shown  to be essential for the activity of the Serratia marcescens nuclease. This residue is located in a conserved region which can be used as a signature pattern and which also contains an aspartic acid residue that could be implicated in the binding of the divalent ion.Expert(s) to contact by email:
July 1999 / Pattern and text revised.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Friedhoof P., Gimadutdinow O., Pingoud A.|
|Title||Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided mutagenesis.|
|Source||Nucleic Acids Res. 22:3280-3287(1994).|