|PROSITE documentation PDOC00856 [for PROSITE entry PS01112]|
In eukaryotes, there are three different forms of DNA-dependent RNA polymerases (EC 22.214.171.124) transcribing different sets of genes. Each class of RNA polymerase is an assemblage of ten to twelve different polypeptides. In archaebacteria, there is generally a single form of RNA polymerase which also consist of an oligomeric assemblage of 10 to 13 polypeptides.
Archaebacterial subunit N (gene rpoN)  is a small protein of about 8 Kd, it is evolutionary related  to a 8.3 Kd component shared by all three forms of eukaryotic RNA polymerases (gene RPB10 in yeast and POLR2J in mammals) as well as to African swine fever virus protein CP80R .
As a signature pattern we selected a conserved region which is located at the N-terminal extremity of these polymerase subunits; this region contains two cysteines that binds a zinc ion .Last update:
May 2004 / Text revised.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Langer D. Hain J. Thuriaux P. Zillig W.|
|Title||Transcription in archaea: similarity to that in eucarya.|
|Source||Proc. Natl. Acad. Sci. U.S.A. 92:5768-5772(1995).|
|2||Authors||McKune K. Woychik N.A.|
|Title||Halobacterial S9 operon contains two genes encoding proteins homologous to subunits shared by eukaryotic RNA polymerases I, II, and III.|
|Source||J. Bacteriol. 176:4754-4756(1994).|
|3||Authors||Yanez R.J. Rodriguez J.M. Nogal M.L. Yuste L. Enriquez C. Rodriguez J.F. Vinuela E.|
|Title||Analysis of the complete nucleotide sequence of African swine fever virus.|
|4||Authors||MacKereth C.D. Arrowsmith C.H. Edwards A.M. McIntosh L.P.|
|Title||Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum.|
|Source||Proc. Natl. Acad. Sci. U.S.A. 97:6316-6321(2000).|