PROSITE documentation PDOC00856
RNA polymerases N / 8 Kd subunits signature


In eukaryotes, there are three different forms of DNA-dependent RNA polymerases (EC transcribing different sets of genes. Each class of RNA polymerase is an assemblage of ten to twelve different polypeptides. In archaebacteria, there is generally a single form of RNA polymerase which also consist of an oligomeric assemblage of 10 to 13 polypeptides.

Archaebacterial subunit N (gene rpoN) [1] is a small protein of about 8 Kd, it is evolutionary related [2] to a 8.3 Kd component shared by all three forms of eukaryotic RNA polymerases (gene RPB10 in yeast and POLR2J in mammals) as well as to African swine fever virus protein CP80R [3].

As a signature pattern we selected a conserved region which is located at the N-terminal extremity of these polymerase subunits; this region contains two cysteines that binds a zinc ion [4].

Last update:

May 2004 / Text revised.


Technical section

PROSITE method (with tools and information) covered by this documentation:

RNA_POL_N_8KD, PS01112; RNA polymerases N / 8 Kd subunits signature  (PATTERN)


1AuthorsLanger D. Hain J. Thuriaux P. Zillig W.
TitleTranscription in archaea: similarity to that in eucarya.
SourceProc. Natl. Acad. Sci. U.S.A. 92:5768-5772(1995).
PubMed ID7597027

2AuthorsMcKune K. Woychik N.A.
TitleHalobacterial S9 operon contains two genes encoding proteins homologous to subunits shared by eukaryotic RNA polymerases I, II, and III.
SourceJ. Bacteriol. 176:4754-4756(1994).
PubMed ID8045907

3AuthorsYanez R.J. Rodriguez J.M. Nogal M.L. Yuste L. Enriquez C. Rodriguez J.F. Vinuela E.
TitleAnalysis of the complete nucleotide sequence of African swine fever virus.
SourceVirology 208:249-278(1995).
PubMed ID11831707

4AuthorsMacKereth C.D. Arrowsmith C.H. Edwards A.M. McIntosh L.P.
TitleZinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum.
SourceProc. Natl. Acad. Sci. U.S.A. 97:6316-6321(2000).
PubMed ID10841539

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