PROSITE documentation PDOC00856RNA polymerases N / 8 Kd subunits signature
In eukaryotes, there are three different forms of DNA-dependent RNA polymerases (EC 2.7.7.6) transcribing different sets of genes. Each class of RNA polymerase is an assemblage of ten to twelve different polypeptides. In archaebacteria, there is generally a single form of RNA polymerase which also consist of an oligomeric assemblage of 10 to 13 polypeptides.
Archaebacterial subunit N (gene rpoN) [1] is a small protein of about 8 Kd, it is evolutionary related [2] to a 8.3 Kd component shared by all three forms of eukaryotic RNA polymerases (gene RPB10 in yeast and POLR2J in mammals) as well as to African swine fever virus protein CP80R [3].
As a signature pattern we selected a conserved region which is located at the N-terminal extremity of these polymerase subunits; this region contains two cysteines that binds a zinc ion [4].
Last update:May 2004 / Text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Langer D. Hain J. Thuriaux P. Zillig W. |
| Title | Transcription in archaea: similarity to that in eucarya. | |
| Source | Proc. Natl. Acad. Sci. U.S.A. 92:5768-5772(1995). | |
| PubMed ID | 7597027 |
| 2 | Authors | McKune K. Woychik N.A. |
| Title | Halobacterial S9 operon contains two genes encoding proteins homologous to subunits shared by eukaryotic RNA polymerases I, II, and III. | |
| Source | J. Bacteriol. 176:4754-4756(1994). | |
| PubMed ID | 8045907 |
| 3 | Authors | Yanez R.J. Rodriguez J.M. Nogal M.L. Yuste L. Enriquez C. Rodriguez J.F. Vinuela E. |
| Title | Analysis of the complete nucleotide sequence of African swine fever virus. | |
| Source | Virology 208:249-278(1995). | |
| PubMed ID | 11831707 |
| 4 | Authors | MacKereth C.D. Arrowsmith C.H. Edwards A.M. McIntosh L.P. |
| Title | Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum. | |
| Source | Proc. Natl. Acad. Sci. U.S.A. 97:6316-6321(2000). | |
| PubMed ID | 10841539 | |
| DOI | 97/12/6316 |
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