PROSITE documentation PDOC00962 [for PROSITE entry PS01249]
Hydrogenases expression/synthesis hypA family signature


Bacterial membrane-bound nickel-dependent hydrogenases requires a number of accessory proteins which are involved in their maturation. The exact role of these proteins is not yet clear, but some seem to be required for the incorporation of the nickel ions [1,2]. One of these proteins is generally known as hypA. It is a protein of about 12 to 14 Kd that contains, in its C-terminal region four conserved cysteines that may bind nickel.

Escherichia coli has two proteins that belong to this family: hypA and hybF. Homolog have also been found in the genome of many archaea.

We developed a signature pattern that starts about 45 residues before the first conserved cysteine and that ends with the second one.

Last update:

April 2006 / Pattern revised.


Technical section

PROSITE method (with tools and information) covered by this documentation:

HYPA, PS01249; Hydrogenases expression/synthesis hypA family signature  (PATTERN)


1AuthorsFu C. Maier R.J.
TitleNucleotide sequences of two hydrogenase-related genes (hypA and hypB) from Bradyrhizobium japonicum, one of which (hypB) encodes an extremely histidine-rich region and guanine nucleotide-binding domains.
SourceBiochim. Biophys. Acta 1184:135-138(1994).
PubMed ID8305450

2AuthorsOlson J.W. Mehta N.S. Maier R.J.
TitleRequirement of nickel metabolism proteins HypA and HypB for full activity of both hydrogenase and urease in Helicobacter pylori.
SourceMol. Microbiol. 39:176-182(2001).
PubMed ID11123699

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