|PROSITE documentation PDOC50199 [for PROSITE entry PS50199]|
Nucleocytoplasmic transport is mediated by large supramolecular structures that span the nuclear envelope called nuclear pore complexes (NPC). The export as well as the import pathway require three major component, the substrate, an adaptor complex and a receptor. The adaptor complex and the receptor pass through the NPC with the substrate. The nuclear transport is also dependent on the GTPase cycle of the small GTPase protein Ran and a number of Ran-binding proteins (RanBP) [1,2].
RanBP2 is one of these proteins located in the NPC on the cytoplasmic side of the ring. The function of RanBP2 is not yet well understood. It could function in coupling RanGTP hydrolysis to NPC translocation through its Ran binding domain (RanBD1) (see <PDOC50196>) [3,4]. RanBP2 contains also a cluster of zinc fingers, the RanBP2 zinc fingers. It has been shown that these zinc fingers bind RanGDP (but not RanGTP)  and the exportin-1 protein (a receptor of the export pathway) .
The RanBP2 zinc finger is also found in different proteins that are mainly involved in nuclear transport or localized to the nuclear envelope. Some of the proteins containing such a domain are listed below.
The RanBP2 zinc finger contains 4 cysteines and a conserved tryptophan upstream of the zinc finger. Our pattern starts with this tryptophan and includes the 4 cysteines. We have also developed a profile that covers a region slightly bigger than the zinc finger.Last update:
May 2002 / Text revised.
PROSITE methods (with tools and information) covered by this documentation:
|1||Authors||Mattaj I.W. Englmeier L.|
|Title||Nucleocytoplasmic transport: the soluble phase.|
|Source||Annu. Rev. Biochem. 67:265-306(1998).|
|2||Authors||Melchior F. Gerace L.|
|Title||Two-way trafficking with Ran.|
|Source||Trends Cell Biol. 8:175-179(1998).|
|3||Authors||Matunis M.J. Coutavas E. Blobel G.|
|Title||A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.|
|Source||J. Cell Biol. 135:1457-1470(1996).|
|4||Authors||Mahajan R. Delphin C. Guan T. Gerace L. Melchior F.|
|Title||A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2.|
|5||Authors||Yaseen N.R. Blobel G.|
|Title||Two distinct classes of Ran-binding sites on the nucleoporin Nup-358.|
|Source||Proc. Natl. Acad. Sci. U.S.A. 96:5516-5521(1999).|
|6||Authors||Singh B.B. Patel H.H. Roepman R. Schick D. Ferreira P.A.|
|Title||The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1.|
|Source||J. Biol. Chem. 274:37370-37378(1999).|