Methylation of cytosine within the dinucleotide CpG is essential for
vertebrate development and is correlated to gene silencing. Sequence analyses
on various mammalian proteins linked to DNA methylation led to the
identification of a conserved cysteine rich region with the consensus sequence
C-x(2)-C-x(2)-C-x(5)-C-x(2)-C-x(2)-C, the CXXC-type zinc finger [1,2]. This
zinc finger can bind specifically to non-methylated CpG DNA but failed to bind
to methylated CpG motifs [3,4].
Some proteins known to contain a CXXC-type zinc finger are listed below:
Mammalian DNA (cytosine-5)-methyltransferase protein 1.
Methyl-CpG binding domain protein 1 (MBD1).
Human Mixed Lineage Leukemia (MLL) protein (also known as all1, htrx, trx1,
or hrx). The MLL gene is a frequent target for chromosomal aberration
associated with leukemia. In the majority of leukemias with altered MLL the
complete C-terminus is removed by chromosomal translocation and replaced in
frame by a variety of different fusion partners. The N-terminal CXXC-type
zinc finger of MLL is essential for the oncongenic activity of various MLL
fusion proteins [4,5].
Human CpG binding protein hCGBP, an activator of genes residing within CpG
The profile we developed covers the whole CXXC-type zinc finger.
January 2005 / First entry.
PROSITE method (with tools and information) covered by this documentation:
Ma Q. Alder H. Nelson K.K. Chatterjee D. Gu Y. Nakamura T. Canaani E. Croce C.M. Siracusa L.D. Buchberg A.M.
Analysis of the murine All-1 gene reveals conserved domains with human ALL-1 and identifies a motif shared with DNA methyltransferases.
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