We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.

PROSITE entry PS51150

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PURL: https://purl.expasy.org/prosite/signature/PS51150

Direct ScanProsite submission

General information about the entry

Entry name [info]	AGOUTI_2
Accession [info]	PS51150
Entry type [info]	MATRIX
Date [info]	01-OCT-2005 CREATED; 10-MAY-2017 DATA UPDATE; 29-MAY-2024 INFO UPDATE.
PROSITE Doc. [info]	PDOC60024
Associated ProRule [info]	PRU00494

Name and characterization of the entry

Description [info]

Agouti domain profile.

Matrix / Profile [info]

/GENERAL_SPEC: ALPHABET='ABCDEFGHIKLMNPQRSTVWYZ'; LENGTH=40;
/DISJOINT: DEFINITION=PROTECT; N1=5; N2=36;
/NORMALIZATION: MODE=1; FUNCTION=LINEAR; R1=2.7000277; R2=0.0052378; TEXT='NScore';
/NORMALIZATION: MODE=-1; FUNCTION=LINEAR; R1=2728.9633789; R2=3.2879581; PRIORITY=1; TEXT='Heuristic 5.0%';
/CUT_OFF: LEVEL=0; SCORE=1108; H_SCORE=6372; N_SCORE=8.5; MODE=1; TEXT='!';
/CUT_OFF: LEVEL=-1; SCORE=726; H_SCORE=5116; N_SCORE=6.5; MODE=1; TEXT='?';
/DEFAULT: M0=-11; D=-50; I=-50; B1=-500; E1=-500; MI=-105; MD=-105; IM=-105; DM=-105;

...

                A   B   C   D   E   F   G   H   I   K   L   M   N   P   Q   R   S   T   V   W   Y   Z
/I:         B1=0; BI=-105; BD=-105;
/M: SY='C';  M=-10,-20,120,-30,-30,-20,-30,-30,-30,-30,-20,-20,-20,-40,-30,-30,-10,-10,-10,-50,-30,-30;
/M: SY='V';  M=  0,-30,-10,-30,-30,  0,-30,-30, 30,-20, 10, 10,-30,-30,-30,-20,-10,  0, 50,-30,-10,-30;
/M: SY='A';  M= 28,-10,-16,-17, -7,-20, -6,-14,-16,  3,-13,-10, -7,-13, -4,  8,  4, -3, -6,-20,-17, -7;
/M: SY='T';  M= -4, -5,-14,-13,-12, -5,-21,-16, -2,-15,  7, -2, -2,-17,-12,-12,  3, 25,  0,-28, -8,-12;
/M: SY='R';  M=-17,-16,-27,-16, -6,-11,-23, -6,-14, 11,  2, -1, -9,-23,  1, 42,-16,-10,-11,-20, -7, -6;
/M: SY='D';  M=-15, 35,-28, 43, 28,-32,-11,  2,-32,  3,-27,-25, 23, -9,  6, -5,  2, -8,-30,-37,-20, 17;
/M: SY='S';  M= 10,  0,-10,  0,  0,-20,  0,-10,-20,-10,-30,-20, 10,-10,  0,-10, 40, 20,-10,-40,-20,  0;
/M: SY='C';  M=-10,-20,120,-30,-30,-20,-30,-30,-30,-30,-20,-20,-20,-40,-30,-30,-10,-10,-10,-50,-30,-30;
/M: SY='K';  M=-10, -9,-27, -9,  1,-17,-23,-13,-14, 25, -5, -1, -9,-16,  1, 14,-16,-10,-11,-20, -7,  1;
/M: SY='P';  M= -4,-14,-32, -8, -6,-28, 11,-18,-26,-13,-30,-20, -9, 40,-12,-18,  1, -9,-27,-28,-28,-12;
/M: SY='P';  M=-13,-14,-37, -7,  0,-27,-20, 18,-23,-10,-27,-14,-11, 55, -4,-14,-10,-13,-30,-30,-14, -7;
/M: SY='A';  M= 31, -7,-16,-14, -1,-26, -6,-11,-13, -4,-13, -7, -7,-10, 12,-11,  7, -3, -9,-20,-17,  6;
/M: SY='P';  M=-10,-24,-35,-22,-12,-16,-27,-23,  7,-19, -5, -3,-21, 41,-14,-23,-15,-10, -6,-26,-17,-17;
/M: SY='A';  M= 31,-13,-19,-17, -7,-23, -6,-20,-13,-10,-16,-13,-13, 22,-10,-20,  4, -3, -9,-23,-23,-10;
/M: SY='C';  M=-10,-20,120,-30,-30,-20,-30,-30,-30,-30,-20,-20,-20,-40,-30,-30,-10,-10,-10,-50,-30,-30;
/M: SY='C';  M=-10,-20,120,-30,-30,-20,-30,-30,-30,-30,-20,-20,-20,-40,-30,-30,-10,-10,-10,-50,-30,-30;
/M: SY='D';  M=-19, 49,-29, 64, 18,-38, -9,  1,-38,  0,-30,-29, 25,-11,  0, -9,  1, -9,-30,-40,-20,  9;
/M: SY='P';  M=-10,-20,-40,-10,  0,-30,-20,-20,-20,-10,-30,-20,-20, 90,-10,-20,-10,-10,-30,-30,-30,-10;
/M: SY='C';  M=-10,-20,120,-30,-30,-20,-30,-30,-30,-30,-20,-20,-20,-40,-30,-30,-10,-10,-10,-50,-30,-30;
/M: SY='A';  M= 50,-10,-10,-20,-10,-20,  0,-20,-10,-10,-10,-10,-10,-10,-10,-20, 10,  0,  0,-20,-20,-10;
/M: SY='S';  M=  2, -5,-12,-10, -8,  0,-11,-15,-13,-13,-17,-13,  2,-13,-10,-12, 24, 24, -5,-28, -8, -8;
/M: SY='C';  M=-10,-20,120,-30,-30,-20,-30,-30,-30,-30,-20,-20,-20,-40,-30,-30,-10,-10,-10,-50,-30,-30;
/M: SY='Q';  M=-13, -6,-30, -6,  7,-18,-23, 13,-14,  4,-14,  0, -6,-16, 38,  4, -6,-10,-24, -4, 18, 21;
/M: SY='C';  M=-10,-20,120,-30,-30,-20,-30,-30,-30,-30,-20,-20,-20,-40,-30,-30,-10,-10,-10,-50,-30,-30;
/M: SY='R';  M=-20,-10,-30,-10,  0,-20,-20,  0,-30, 30,-20,-10,  0,-20, 10, 70,-10,-10,-20,-20,-10,  0;
/M: SY='F';  M=-20,-30,-20,-40,-30, 80,-30,-20,  0,-30, 10,  0,-20,-30,-40,-20,-20,-10,  0, 10, 30,-30;
/M: SY='F';  M=-20,-30,-20,-40,-30, 80,-30,-20,  0,-30, 10,  0,-20,-30,-40,-20,-20,-10,  0, 10, 30,-30;
/M: SY='R';  M=-14,  6,-27, -1, -3,-22,  1,  0,-28, 13,-25,-15, 19,-20,  2, 33, -2, -8,-25,-26,-16, -3;
/M: SY='S';  M= 23, -3,-10, -6, -3,-20,  0,-13,-17,-10,-24,-17,  4,-10, -3,-13, 31, 14, -7,-34,-20, -3;
/M: SY='A';  M= 26,-16,-13,-25,-16, 12, -9,-19, -7,-16, -5, -7,-12,-16,-19,-19,  2, -2, -1,-12, -4,-16;
/M: SY='C';  M=-10,-20,120,-30,-30,-20,-30,-30,-30,-30,-20,-20,-20,-40,-30,-30,-10,-10,-10,-50,-30,-30;
/M: SY='T';  M= -3, -6,-16, -9, -9, -1,-16, -4,-11,-10,-14,-11, -3,-16, -6,-10, 15, 20, -7,-15, 15, -9;
/M: SY='C';  M=-10,-20,120,-30,-30,-20,-30,-30,-30,-30,-20,-20,-20,-40,-30,-30,-10,-10,-10,-50,-30,-30;
/M: SY='R';  M=-20,-10,-30,-10,  0,-20,-20,  0,-30, 30,-20,-10,  0,-20, 10, 70,-10,-10,-20,-20,-10,  0;
/M: SY='V';  M= -3,-21,-16,-21,-17, -9,-27,-24, 11,  2, -3,  4,-21,-24,-17, -4,-10, -3, 28,-27,-10,-17;
/M: SY='L';  M=-10,-30,-23,-33,-23,  7,-33,-23, 29,-30, 41, 20,-27,-27,-20,-23,-27,-10, 16,-20,  0,-23;
/M: SY='N';  M=  2, 16,-14,  7, -1,-19, -1, -3,-19, -6,-29,-19, 29,-14, -1, -6, 27, 14,-17,-39,-19, -1;
/M: SY='P';  M= -8,-14,-28,-11, -5,-20,-21,-19,-15, -9,-17,-13,-13, 42, -9,-11, -2,  9,-17,-29,-20,-10;
/M: SY='T';  M= -3,  9,-14, -2, -6,-14,-14,-11,-15, -5,-17,-13, 15,-13, -5, -2, 17, 31, -9,-32,-13, -6;
/I:         I=-14; MI=0; MD=-29; IM=0; DM=-29;
/M: SY='C';  M=-10,-20,120,-30,-30,-20,-30,-30,-30,-30,-20,-20,-20,-40,-30,-30,-10,-10,-10,-50,-30,-30;
/I:         E1=0; IE=-105; DE=-105;

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Numerical results [info]

Numerical results for UniProtKB/Swiss-Prot release 2025_04 which contains 573'661 sequence entries.

Total number of hits	48 in 48 different sequences
Number of true positive hits	48 in 48 different sequences
Number of 'unknown' hits	0
Number of false positive hits	0
Number of false negative sequences	10
Number of 'partial' sequences	0

Precision (true positives / (true positives + false positives))	100.00 %
Recall (true positives / (true positives + false negatives))	82.76 %

Comments [info]

Taxonomic range [info]	Eukaryotes
Maximum number of repetitions [info]	1
Matrix type [info]	protein_domain
Scaling database [info]	reversed
Author [info]	CJA_Sigrist
Feature key [info]	DOMAIN
Feature description [info]	Agouti
Version [info]	5

Cross-references [info]

UniProtKB/Swiss-Prot
True positive sequences

48 sequences

AGRP_BOVIN  (P56413), AGRP_HUMAN  (O00253), AGRP_MOUSE  (P56473),

AGRP_PIG(Q9TU18), ASIP_BOVIN  (Q29414), ASIP_CALGE  (A1YL78), 
ASIP_CALGO  (A1YL80), ASIP_CALJA  (A1YL77), ASIP_CANLF  (Q5UK76), 
ASIP_CEBPY  (A1YL79), ASIP_CERMI  (Q1XGU9), ASIP_CHLAE  (Q1XGV1), 
ASIP_COLPO  (Q1XGU5), ASIP_ERYPA  (Q1XGV0), ASIP_FELCA  (Q865F0), 
ASIP_GORGO  (Q1XGV5), ASIP_HORSE  (Q95MP2), ASIP_HUMAN  (P42127), 
ASIP_LEOCY  (A1YL81), ASIP_LEORO  (A1YL82), ASIP_MACAR  (A8CEM9), 
ASIP_MACAS  (A8CEN3), ASIP_MACCY  (A8CEM1), ASIP_MACFA  (Q1XGV3), 
ASIP_MACFU  (A8CEM0), ASIP_MACHE  (A8CEM5), ASIP_MACMR  (A8CEM4), 
ASIP_MACMU  (A1YL67), ASIP_MACNE  (A1YL69), ASIP_MACNG  (A1YL68), 
ASIP_MACNR  (A8CEM7), ASIP_MACRA  (Q1XGV2), ASIP_MACSI  (A8CEN1), 
ASIP_MACSL  (A8CEM8), ASIP_MACSY  (A1YL66), ASIP_MOUSE  (Q03288), 
ASIP_PANPA  (Q1XGV6), ASIP_PANTR  (Q1XGV7), ASIP_PAPAN  (A1YL70), 
ASIP_PIG(Q6ZYM3), ASIP_PONPY  (Q1XGV4), ASIP_RAT(Q99JA2), 
ASIP_SEMEN  (Q1XGU8), ASIP_TRAAU  (A1YL72), ASIP_TRACR  (Q1XGU6), 
ASIP_TRAFR  (A1YL74), ASIP_TRAOB  (Q1XGU7), ASIP_VULVU  (P79407)

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Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
[Clustal format, color, condensed view] [Clustal format, color]
[Clustal format, plain text] [Fasta format]
Retrieve the sequence logo from the alignment

UniProtKB/Swiss-Prot
False negative sequences

10 sequences

TXD01_LYCSI (B6DD19), TXD02_LYCSI (B6DD20), TXD03_LYCSI (B6DD21),

TXD04_LYCSI (B6DD22), TXD05_LYCSI (B6DD23), TXD06_LYCSI (B6DD24), 
TXD07_LYCSI (B6DD25), TXD08_LYCSI (B6DD26), TXD09_LYCSI (B6DD27), 
TXD10_LYCSI (B6DD28)

» more

PDB
[Detailed view]

6 PDB

pdb_00001hyk; pdb_00001mr0; pdb_00001y7j; pdb_00001y7k; pdb_00002kza; pdb_00002l1j