|PROSITE documentation PDOC51802 [for PROSITE entry PS51802]|
Zinc finger (ZF) proteins are a large family of metalloproteins that utilize zinc for structural purposes. Zinc coordinates to a combination of cysteine thiol and histidine imidazole residues within the ZF polypetide sequence resulting in a folded and functional protein. The CCHHC family of ZFs is a small family of "nonclassical" ZFs that are essential for the development of the central nervous system. These proteins have up to seven putative zinc-binding sequences, usually arranged in sets of two or more sequences. The CCHHC-type zinc finger is able to bind specific double-stranded DNA sequences [1,2,3,4,5].
The CCHHC-type zinc finger contains five absolutely conserved cysteine and histidine residues (rather than the more usual four) with the sequence C-P-x-P-G-C-x-G-x-G-H-x(7)-H-R-x(4)-C. The second histidine has been shown to coordinate Zn(II) along with the three cysteines residues. The first His plays a different role in stabilizing the structure, stacking between the metal-binding core and an aromatic residue that is relatively conserved within this domain family. CCHHC-type zinc fingers form small compact structures that can sit entirely within the major groove of DNA (see <PDB:2JX1>) [1,2,3,4,5].
Some proteins known to contain a CCHHC-type zinc finger are listed below:
The profile we developed covers the entire CCHHC-type zinc finger.Last update:
June 2016 / First entry.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Gamsjaeger R. Swanton M.K. Kobus F.J. Lehtomaki E. Lowry J.A. Kwan A.H. Matthews J.M. Mackay J.P.|
|Title||Structural and biophysical analysis of the DNA binding properties of myelin transcription factor 1.|
|Source||J. Biol. Chem. 283:5158-5167(2008).|
|2||Authors||Gamsjaeger R. O'Connell M.R. Cubeddu L. Shepherd N.E. Lowry J.A. Kwan A.H. Vandevenne M. Swanton M.K. Matthews J.M. Mackay J.P.|
|Title||A structural analysis of DNA binding by myelin transcription factor 1 double zinc fingers.|
|Source||J. Biol. Chem. 288:35180-35191(2013).|
|3||Authors||Lee S.J. Michel S.L.J.|
|Title||Structural metal sites in nonclassical zinc finger proteins involved in transcriptional and translational regulation.|
|Source||Acc. Chem. Res. 47:2643-2650(2014).|
|4||Authors||Besold A.N. Michel S.L.|
|Title||Neural Zinc Finger Factor/Myelin Transcription Factor Proteins: Metal Binding, Fold, and Function.|
|5||Authors||Berkovits-Cymet H.J. Amann B.T. Berg J.M.|
|Title||Solution structure of a CCHHC domain of neural zinc finger factor-1 and its implications for DNA binding.|