PROSITE documentation PDOC52036 [for PROSITE entry PS52037]Zinc finger reverse gyrase (RG) N-terminal- and C-terminal-type profiles
Regulation of DNA topology is central to a multitude of cellular events, including gene expression, DNA replication, recombination and repair. DNA topoisomerases inter-convert topoisomers by introducing or removing positive or negative supercoils. Reverse gyrase (RG) is an ATP-dependent topoisomerase that is only found in archaeal and bacterial hyperthermophiles (above 80 degrees C) and thermophiles (65-80 degrees C). It can protect DNA from damage at high temperatures as a heat-protective DNA chaperone or DNA renaturase and it is also involved in protecting DNA against physical or chemical damage. RGs share a modular structure with an N-terminal cysteine-rich region (a zinc finger) preceding a helicase domain that is followed by a C-terminal topoisomerase domain. The helicase domain is subdivided into H1 and H2 domains that are flexibly linked. Some RG topoisomerase domains also include a cysteine-rich region that has been proposed to form a second zinc finger. The two zinc-finger motifs are found to be important to the structure stability maintenance of RG at high temperature. The N-terminal zinc finger firmly attaches the H1 domain to the topoisomerase domain and may contribute to double-strand DNA (dsDNA) binding. The second zinc finger locates to the topoisomerase domain at a position close to the single-strand DNA-binding site [1,2].
The N-terminal cysteine-rich region folds into a compact domain that harbors a Gag-knuckle zinc finger. A β-hairpin called a zinc-knuckle carries two cysteine residues at its tip and two more zinc ligands are provided by a short α-helix or loop region. The C-terminal cysteine-rich region in RG forms a zinc finger of the ribbon type with two zinc knuckles clamping onto the zinc ion. The zinc knuckles are joined by an additional β-strand to form a three-stranded anti-parallel β-sheet. The zinc ribbon in RG is inserted into the Toprim domain (see <PDOC50880>) at the base of the DNA-binding site and protrudes from the surface of the topoisomerase domain (see <PDB:4DDX>) [1].
The profiles we developed cover respectively the entire RG N-terminal- and C-terminal-type zinc fingers.
Last update:September 2023 / First entry.
-------------------------------------------------------------------------------
PROSITE methods (with tools and information) covered by this documentation:
| 1 | Authors | Rudolph M.G. del Toro Duany Y. Jungblut S.P. Ganguly A. Klostermeier D. |
| Title | Crystal structures of Thermotoga maritima reverse gyrase: inferences for the mechanism of positive DNA supercoiling. | |
| Source | Nucleic. Acids. Res. 41:1058-1070(2013). | |
| PubMed ID | 23209025 | |
| DOI | 10.1093/nar/gks1073 |
| 2 | Authors | Li J. Liu J. Zhou J. Xiang H. |
| Title | Functional evaluation of four putative DNA-binding regions in Thermoanaerobacter tengcongensis reverse gyrase. | |
| Source | Extremophiles 15:281-291(2011). | |
| PubMed ID | 21318561 | |
| DOI | 10.1007/s00792-011-0356-5 |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
View entry in original PROSITE document format
View entry in raw text format (no links)