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ProRule PRU00392


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PURL: https://purl.expasy.org/prosite/rule/PRU00392
General rule information [?]

Accession PRU00392
Dates 04-FEB-2005 (Created)
11-JAN-2024 (Last updated, Version 18)
Data class Domain;
Predictors PROSITE; PS51068; FPG_CAT
Name Formamidopyrimidine-DNA glycosylase catalytic domain
Function DNA glycosylase
Scope(s) Eukaryota
Bacteria
Archaea
Example(s) P05523;

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1>
Protein name + AltName: EC=3.2.2.-;
                 EC=4.2.99.18;

Comments [?]

FUNCTIONInvolved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases.
CATALYTIC ACTIVITY Reaction=2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'- deoxyribonucleoside-DNA + H(+); Xref=Rhea:RHEA:66592, Rhea:RHEA- COMP:13180, Rhea:RHEA-COMP:16897, Rhea:RHEA-COMP:17067, ChEBI:CHEBI:15378, ChEBI:CHEBI:136412, ChEBI:CHEBI:157695, ChEBI:CHEBI:167181; EC=4.2.99.18;
SIMILARITYBelongs to the FPG family.
end case

Keywords [?]

case <FTGroup:1>
DNA damage
DNA repair
DNA-binding
Glycosidase
Hydrolase
Lyase
Multifunctional enzyme
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0006974; Biological process:DNA damage response
GO:0006281; Biological process:DNA repair
GO:0003677; Molecular function:DNA binding
GO:0016798; Molecular function:hydrolase activity, acting on glycosyl bonds
GO:0016787; Molecular function:hydrolase activity
GO:0016829; Molecular function:lyase activity
GO:0003824; Molecular function:catalytic activity
end case

Features [?]

From: PS51068
Key From To Description Tag Condition FTGroup
ACT_SITE 1 1 /note="Schiff-base intermediate with DNA" P 1
ACT_SITE 2 2 /note="Proton donor" E 1
ACT_SITE 57 57 /note="Proton donor (in beta-elimination)" K 1

Additional information [?]

Size range 70-150 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]