PROSITE logo
Black ribbon
We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU00392


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00392
General rule information [?]

Accession PRU00392
Dates 04-FEB-2005 (Created)
11-JAN-2024 (Last updated, Version 18)
Data class Domain;
Predictors PROSITE; PS51068; FPG_CAT
Name Formamidopyrimidine-DNA glycosylase catalytic domain
Function DNA glycosylase
Scope(s) Eukaryota
Bacteria
Archaea
Example(s) P05523;

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1>
Protein name + AltName: EC=3.2.2.-;
                 EC=4.2.99.18;

Comments [?]

FUNCTIONInvolved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases.
CATALYTIC ACTIVITY Reaction=2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'- deoxyribonucleoside-DNA + H(+); Xref=Rhea:RHEA:66592, Rhea:RHEA- COMP:13180, Rhea:RHEA-COMP:16897, Rhea:RHEA-COMP:17067, ChEBI:CHEBI:15378, ChEBI:CHEBI:136412, ChEBI:CHEBI:157695, ChEBI:CHEBI:167181; EC=4.2.99.18;
SIMILARITYBelongs to the FPG family.
end case

Keywords [?]

case <FTGroup:1>
DNA damage
DNA repair
DNA-binding
Glycosidase
Hydrolase
Lyase
Multifunctional enzyme
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0006974; Biological process:DNA damage response
GO:0006281; Biological process:DNA repair
GO:0003677; Molecular function:DNA binding
GO:0016798; Molecular function:hydrolase activity, acting on glycosyl bonds
GO:0016787; Molecular function:hydrolase activity
GO:0016829; Molecular function:lyase activity
GO:0003824; Molecular function:catalytic activity
end case

Features [?]

From: PS51068
Key From To Description Tag Condition FTGroup
ACT_SITE 1 1 /note="Schiff-base intermediate with DNA" P 1
ACT_SITE 2 2 /note="Proton donor" E 1
ACT_SITE 57 57 /note="Proton donor (in beta-elimination)" K 1

Additional information [?]

Size range 70-150 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]