We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
ProRule PRU00408
View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU00408
General rule information
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| Accession | PRU00408 |
| Dates | 03-MAR-2005 (Created)
19-NOV-2022 (Last updated, Version 20) |
| Data class | Domain; |
| Predictors |
PROSITE; PS50879; RNASE_H_1 |
Name | Ribonuclease H type-1 domain |
| Function | Ribonuclease H (RNase H) (EC 3.1.26.4) recognizes and cleaves the RNA strand of RNA-DNA heteroduplexes. |
| Scope(s) |
Eukaryota Bacteria Archaea Viruses |
| Example(s) | Q08885; |
Propagated annotation
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Identifier, protein and gene names
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| Protein name | + RecName: EC=3.1.26.4; |
Comments
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| CATALYTIC ACTIVITY | Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.4; |
Keywords
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| Endonuclease | |
| Hydrolase | |
| Nuclease | |
| case <FTGroup:1> | |
| Magnesium | |
| Metal-binding | |
| end case | |
Gene Ontology
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| GO:0004519; Molecular function:endonuclease activity | |
| GO:0016787; Molecular function:hydrolase activity | |
| GO:0004518; Molecular function:nuclease activity | |
| case <FTGroup:1> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
Features
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| From: PS50879 | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | from | to | /note="RNase H type-1" | |||||||||
| case not <OC:Bacteria> and not <OC:Archaea> | ||||||||||||
| BINDING | 10 | 10 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="#1" |
D | 1 | |||||||
| BINDING | 10 | 10 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="#2" |
D | 1 | |||||||
| BINDING | 50 | 50 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="#1" |
E | 1 | |||||||
| BINDING | 72 | 72 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="#1" |
D | 1 | |||||||
| BINDING | 136 | 136 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="#2" |
D | 1 | |||||||
| end case | ||||||||||||
Additional information
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| Size range | 101-205 amino acids |
| Related rules |
None |
| Fusion | None |
| Repeats | 1 |
| Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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